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CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells | |
Liu, Bingtao1,2,3,4; Li, Hongbin5; Liu, Xiongxiong1,2,3,4; Li, Feifei6; Chen, Weiqiang1,2,3,4; Kuang, Yanbei1,2,3,4; Zhao, Xueshan7; Li, Linying1,2,3,4; Yu, Boyi1,2,3,4; Jin, Xiaodong1,2,3,4 | |
2021-08 | |
发表期刊 | Cell Signal |
ISSN | 0898-6568 |
卷号 | 84期号:84页码:110012 |
摘要 | Background: Mounting evidence suggests that circular RNAs (circRNAs) are closely related to the regulation of gene expression during tumour development. However, the role of circRNAs in modulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells has not been explored. Methods: Transcriptome sequencing was used to explore the expression profiles of circRNAs in NSCLC. The expression level of circRNAs was changed by inducing instantaneous knockdown or overexpression. Changes in proliferation and radiosensitivity of NSCLC cells were investigated using CCK-8, EDU, and clonal survivals. Results: By analysing the circRNA expression profile of NSCLC cells, we found that circRNA ZNF208 (circZNF208) was significantly upregulated in a radioresistant NSCLC cell line (A549-R11), which was acquired from the parental NSCLC cell line A549. Knockout experiments indicated that circZNF208 enhanced the radiosensitivity of A549 and A549-R11 cells to X-rays. Mechanistically, circZNF208 upregulated SNCA expression by acting as a sponge of miR-7-5p and subsequently promoted the resistance of NSCLC cells to low linear energy transfer (LET) X-rays. However, this effect was not observed in NSCLC cells exposed to high-LET carbon ions. Conclusions: Knockdown of circZNF208 altered the radiosensitivity of patients with NSCLC to X-rays but did not significantly change the sensitivity to carbon ions. Therefore, circZNF208 might serve as a potential biomarker and therapeutic target for NSCLC treatment with radiotherapy of different modalities. |
关键词 | Non-small cell lung cancer Radio-sensitivity SNCA circZNF208 miR-7-5p |
学科领域 | 基础医学 |
学科门类 | 医学 |
DOI | 10.1016/j.cellsig.2021.110012 |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
WOS研究方向 | Cell Biology |
WOS类目 | Cell Biology |
WOS记录号 | WOS:000657674900005 |
出版者 | ELSEVIER SCIENCE INC |
来源库 | WOS |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/148470 |
专题 | 生命科学与工程学院 |
通讯作者 | Jin, Xiaodong; Li, Qiang |
作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China; 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China; 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 6.Northwest Normal Univ, Lanzhou, Gansu, Peoples R China; 7.Lanzhou Univ, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Bingtao,Li, Hongbin,Liu, Xiongxiong,et al. CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells[J]. Cell Signal,2021,84(84):110012. |
APA | Liu, Bingtao.,Li, Hongbin.,Liu, Xiongxiong.,Li, Feifei.,Chen, Weiqiang.,...&Li, Qiang.(2021).CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells.Cell Signal,84(84),110012. |
MLA | Liu, Bingtao,et al."CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells".Cell Signal 84.84(2021):110012. |
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