甜菜内向整流K+通道BvAKT1基因RNAi载体的构建
伍国强; 刘海龙; 李胜胜; 蔺丽媛; 谢玲玲
2019
Source Publication分子植物育种
ISSN1672-416X
Volume17Issue:1Pages:112-118
Abstract植物细胞质中维持较高的K~+/Na~+是植物抵御盐胁迫的有效策略之一。内向整流K~+通道AKT1(Arabidopsis K~+ transporter)在这一过程中发挥着重要作用。本研究以嗜盐作物甜菜(Beta vulgaris L.)幼苗为材料,提取其根总RNA,采用RT-PCR方法扩增得到甜菜K~+通道BvAKT1基因的RNAi靶片段,以中间载体 pHANNIBAL和植物表达载体pART27为基础,采用酶切和连接的方法构建了由CaMV 35S启动子驱动、含 BvAKT1基因片段反向重复序列的RNAi(RNA interference)植物表达载体pART-BvAKT1-RNAi,采用冻融法将其分别导入根癌农杆菌GV3101和发根农杆菌LBA9402,对揭示BvAKT1在甜菜K~+吸收及离子稳态平衡中的作用研究具有推动作用。
Keyword甜菜 内向整流K+通道 K+吸收 RNA干扰
Indexed ByCSCD
Language中文
WOS Research AreaAgriculture
WOS SubjectAGRONOMY
CSCD IDCSCD:6412393
Document Type期刊论文
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/74474
Collection生命科学与工程学院
Affiliation兰州理工大学生命科学与工程学院, 兰州, 甘肃 730050, 中国
First Author AffilicationColl Life & Sci Engn
First Signature AffilicationColl Life & Sci Engn
Recommended Citation
GB/T 7714
伍国强,刘海龙,李胜胜,等. 甜菜内向整流K+通道BvAKT1基因RNAi载体的构建[J]. 分子植物育种,2019,17(1):112-118.
APA 伍国强,刘海龙,李胜胜,蔺丽媛,&谢玲玲.(2019).甜菜内向整流K+通道BvAKT1基因RNAi载体的构建.分子植物育种,17(1),112-118.
MLA 伍国强,et al."甜菜内向整流K+通道BvAKT1基因RNAi载体的构建".分子植物育种 17.1(2019):112-118.
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