极端嗜酸硫杆菌在含砷难处理金矿的氧化预处理过程中的抗砷机制研究
Project Number31460032
冷非凡
Abstract砷对生物体具有较明显的毒害作用,许多生物仅能耐受较低浓度的砷。自然环境中的砷多数与金等矿石伴生,含砷难处理金矿中含有的大量砷阻碍了多数浸矿微生物的正常生长和对矿石的氧化预处理。目前,除了在大肠杆菌等微生物中证实了arsRDABC和arsRBC等典型的砷抗性操纵子以外,还陆续在能耐受较高浓度砷的菌株中发现了一些新的抗砷基因和抗砷机制。关于极端嗜酸硫杆菌在微生物浸矿中的抗砷行为和机制的报道为数不多,尚未见关于嗜酸氧化硫硫杆菌抗砷机制的报道,微生物的抗砷机制至今仍未被完全解释。在我们的前期工作中,分离得到了若干株嗜酸氧化亚铁硫杆菌和嗜酸氧化硫硫杆菌,研究发现它们的抗砷浓度超过了目前已有报道的所有浸矿微生物。在本项目中,拟通过对比不同来源的抗性菌株之间抗砷机理的差异,考证传统的砷抗性操纵子、转座子模型的保守性以及是否存在新的抗砷基因及抗砷机制。【英文摘要】Arsenichasobvioustoxiceffecttoorganisms.Manyorganismscanonlytolerateverylowdosageofarsenic.Arsenicintheenvironmentisalwaysfoundtobeassociatedwithseveralsulfidemineralslikegoldore.However,highconcentrationofarseniccompoundsexistedinthearsenic-bearingrefractorygoldoresinhibitsgrowthofalargenumberofbioleachingmicroorganismsandimpedestheoxidationpretreatmentsofores.Untilnow,somenovelarsenicresistancegenesandmechanismshavebeenprovedtoplaykeyrolesincertainmicroorganismstrainswhichcouldresisthighconcentrationofarsenic,besidesthosetypicalarsenicresistanceoperonssuchasarsRDABCandarsRBCfoundinEscherichiacoliandothercommonstrains.Ontheotherhand,therehavebeenonlyafewreportsaboutarsenicresistancebehaviorsandmechanismsofAcidithiobacillusspp.inthebioleaching,andnopaperonarsenicresistancemechanismsofAcidithiobacillusthiooxidanshasbeenreported.Therefore,arsenicresistancemechanismsofmicroorganismshavenotbeenilluminatedcompletely.Inourpreviouswork,severalstrainsofAcidithiobacillusferrooxidansandAcidithiobacillusthiooxidansweresuccessfullyisolatedandapplicatedintheoxidationpretreatmentsofarsenic-bearingrefractorygoldores,anditwasdemonstratedthatthearsenicresistanceconcentrationswerehigherthanthoseofallthebioleachingmicroorganismsreported.Inthisstudy,differencesbetweenarsenicresistancemechanismsfromdifferentsourceswillbecomparedamongarsenicresistancebehaviorsandmechanismsofseveralresistantstrainsofAcidithiobacillusspp.Furthermore,thehereditaryconservationsofarsoperonandtransposons,andwhethertherearesomeunknownarsenicresistancegenesormechanismswillbeexamined.
Subtype地区科学基金项目
Project Source国家自然科学基金
2015-01
End Date2018-12
MOST Discipline Catalogue06 - 生物科学 ; 0601 - 微生物学
Host Institution兰州理工大学
Project Funding510000.0
CountryCN
Language中文
Document Type项目
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/64748
Collection生命科学与工程学院
Recommended Citation
GB/T 7714
冷非凡.极端嗜酸硫杆菌在含砷难处理金矿的氧化预处理过程中的抗砷机制研究.2015.
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