Na+提高甜菜抗旱性的作用机制研究
Project Number31460101
伍国强
Abstract甜菜是一种抗逆性较强的嗜钠作物,以往有关Na+对甜菜有益作用的研究多集中在生长和耐盐性上,而我们最近发现Na+在甜菜响应干旱胁迫过程中也发挥着重要作用;液泡膜Na+/H+逆向转运蛋白(NHX)是介导液泡Na+区域化的重要功能蛋白,但其在甜菜抗旱性中的作用机理尚不清楚。本项目拟在前期工作基础上,通过系统分析渗透胁迫下2个抗旱性不同的甜菜品种根和叶细胞中Na+微区分布,探明甜菜Na+区域化与抗旱性的关系;克隆甜菜NHX蛋白基因BvNHX,采用Real-timePCR技术分析该基因在渗透胁迫下的转录丰度,通过在抗旱性强的品种中沉默BvNHX基因的表达、在抗旱性弱的品种中超表达该基因,分析沉默植株和超表达植株Na+微区分布及抗旱性的变化。以期揭示Na+在甜菜适应干旱中的作用机理,为阐明嗜钠作物逆境适应机制奠定理论基础。对我国北方地区农作物的抗逆性遗传改良和生态环境建设具有重要理论意义和实践价值。【英文摘要】Sugarbeet(BetavulgarisL.)isconsideredasanatrophiliccropwiththestrongerresistancetoadverseenvironment.ResearchesonbeneficialeffectsofNa+insugarbeetwerelimitedmainlythegrowthandsalttolerance,however,ourrecentstudiesshowedthatNa+alsoplaysveryimportantrolesintheresponseofsugarbeetplantstodroughtstress.TonoplastNa+/H+antiporter(NHX)provedtobeinvolvedinthecompartmentationofNa+intovacuolesfromcytosol.However,theprecisemechanismsunderlyingthistransporterindroughttoleranceofsugarbeetremainedunclear.Thisproject,basedonourpreviousstudies,willanalyzetheintracellularmicro-distributionofNa+inbothrootandshootoftwosugarbeetcultivarswithdifferingdroughttoleranceunderosmoticstress,toinvestigaterelationshipbetweenNa+compartmentationanddroughttolerance.BvNHXgeneencodingtonoplastNa+/H+antiporterwillbeclonedandcharacterizedinsugarbeet;thetranscriptabundanceofthisgenewillbequantitativelyanalyzedbyusingreal-timePCRintwocultivarsexposedtoosmoticstress;BvNHXgeneindrought-resistancecultivarwillbesilencedbyRNAinterferencemethod,andthisgenewillalsooverexpressedindrought-sensitiveone,thencellularmicro-distributionofNa+andthechangesofdroughttolerancewillbetestedinbothBvNHX-silencedplantsandBvNHX-overexpressedplants,respectively.ResultsofthisprojectmayrevealthemechanismsunderlyingNa+inadaptationofsugarbeettodrought,andalsoshouldprovidethetheoreticalbasisforelucidatingadaptativemechanismsofnatrophiliccropstoadverseenvironment.Overall,thisprojecthastheimportanttheorysignificanceandthepracticevalueforthegeneticimprovementofcropsandprotectionofecologicalenvironmentinaridandsemi-aridregionsinNorthChina.
Subtype地区科学基金项目
Project Source国家自然科学基金
2015-01
End Date2018-12
MOST Discipline Catalogue06 - 生物科学 ; 0603 - 生态学
Host Institution兰州理工大学
Project Funding500000.0
CountryCN
Language中文
Document Type项目
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/64747
Collection生命科学与工程学院
Recommended Citation
GB/T 7714
伍国强.Na+提高甜菜抗旱性的作用机制研究.2015.
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