The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops

doi:10.1016/j.foodcont.2022.109048

	The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops
	Wang, Jinbin 1,2; Wang, Yu1,3 ; Hu, Xiuwen 1; Yang, Qianwen 1; Chen, Yifan 1,2; Jiang, Wei 1,2; Liu, Xiaofeng1,3 ; Liu, Hua 1,2; Zeng, Haijuan 1,2
	2022-09
发表期刊	FOOD CONTROL
ISSN	0956-7135
卷号	139
摘要	Rapid detection method with high sensitivity has been expected in transgenic field screening. In this study, an ultra-sensitive visualization detection procedure based on recombinase polymerase amplification, CRISPR/ Cas12a system, and lateral flow strip technology (RPA-Cas12a-LFB) was developed for the rapid detection of genetically modified (GM) crops. P-CaMV 35 S and T -NOS screening elements, the most widely covered in GM crops, were targeted. The results showed that standard plasmids as low as 10 copies and 0.01% GM crops of Bt11 and MON863 samples can be detected, demonstrating the ultra-sensitivity of the method. The assay was applied to a series of GM crop standards, and the results were consistent with those of existing methods, exhibiting the excellent specificity and feasibility. The analysis can be completed within 40 min, realizing rapid detection. Overall, an accurate, sensitive, time-saving, and instrument-independent detection system was successfully developed, providing a new strategy with ultra-sensitive capability in detection.
关键词	Genetically modified crops Lateral flow test strip CRISPR Cas12a On-site detection
DOI	10.1016/j.foodcont.2022.109048
收录类别	SCIE
语种	英语
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:000799020100004
出版者	ELSEVIER SCI LTD
来源库	WOS
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/158923
专题	能源与动力工程学院生命科学与工程学院
通讯作者	Zeng, Haijuan
作者单位	1.Shanghai Acad Agr Sci, Inst Biotechnol Res, Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China; 2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspection & Supervis Ct, 2901 Beidi Rd, Shanghai 201106, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinbin,Wang, Yu,Hu, Xiuwen,et al. The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops[J]. FOOD CONTROL,2022,139.
APA	Wang, Jinbin.,Wang, Yu.,Hu, Xiuwen.,Yang, Qianwen.,Chen, Yifan.,...&Zeng, Haijuan.(2022).The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops.FOOD CONTROL,139.
MLA	Wang, Jinbin,et al."The development of RPA and CRISPR-Cas12a based immunoassay strip for sensitive detection of genetically modified crops".FOOD CONTROL 139(2022).