The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field-stimulated osteoblastic differentiation

doi:10.1096/fj.202101577RR

	The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field-stimulated osteoblastic differentiation
	He, Wen-Fang 1,2,3; Qin, Rong 1; Gao, Yu-Hai 1; Zhou, Jian 1; Wei, Juan-Juan 1; Liu, Jing 1; Hou, Xue-Feng 1; Ma, Hui-Ping 1; Xian, Cory J.4; Li, Xue-Yan 2
	2022-06
发表期刊	FASEB JOURNAL
ISSN	0892-6638
卷号	36 期号:6
摘要	Pulsed electromagnetic fields (PEMFs) have long been recognized being safe and effective in treating bone fracture nonunion and osteoporosis. However, the mechanism of osteogenic action of PEMFs is still unclear. While primary cilia are reported to be a sensory organelle for PEMFs, and nitric oxide (NO) plays an indispensable role in osteogenic effect of PEMFs, the relationship between NO and primary cilia is unknown. In this study, effects of treatment with 50 Hz 0.6 mT PEMFs on osteogenic differentiation and mineralization, NO secretion, and ciliary location of specific proteins were examined in rat calvarial osteoblasts (ROBs) with normal or abrogated primary cilia. It was found that PEMFs stimulated the osteogenic differentiation by activating the NOS/NO/sGC/cGMP/PKG signaling pathway, which need the existence of primary cilia. All components of the signaling pathway including iNOS, eNOS, sGC, PKG-1, and PKG-2 were localized to primary cilia, and eNOS was phosphorylated inside the primary cilia. Besides, primary cilia were elongated significantly by PEMF treatment and changed dynamically with the activation NO/cGMP pathway. When the pathway was blocked by L-NAME, PEMFs could no longer elongate the primary cilia and stimulate the osteoblastic differentiation. Thus, this study for the first time observed activation of the NO/cGMP signaling pathway in ciliary compartment of osteoblasts, and PEMFs could not stimulate the osteoblastic differentiation if the NO signaling pathway was blocked or the ciliogenesis was inhibited. Our findings indicate the interdependent relationship between NO and primary cilia in the PEMF-promoted osteogenesis.
关键词	endothelial nitric oxide synthase nitric oxide osteogenic differentiation primary cilia pulsed electromagnetic fields
DOI	10.1096/fj.202101577RR
收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biology ; Cell Biology
WOS记录号	WOS:000800257900001
出版者	WILEY
来源库	WOS
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/158687
专题	兰州理工大学
通讯作者	Li, Xue-Yan; Chen, Ke-Ming
作者单位	1.940th Hosp Joint Logist Support Force, Peoples Liberat Army China, Fundamental Med Sci Res Labs, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Dept Bioengn, Lanzhou, Peoples R China; 3.Key Lab Stem Cells & Gene Drugs Gansu Prov, Lanzhou, Peoples R China; 4.Univ South Australia, UniSA Clin & Hlth Sci, Adelaide, SA, Australia
第一作者单位	生命科学与工程学院
通讯作者单位	生命科学与工程学院
推荐引用方式 GB/T 7714	He, Wen-Fang,Qin, Rong,Gao, Yu-Hai,et al. The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field-stimulated osteoblastic differentiation[J]. FASEB JOURNAL,2022,36(6).
APA	He, Wen-Fang.,Qin, Rong.,Gao, Yu-Hai.,Zhou, Jian.,Wei, Juan-Juan.,...&Chen, Ke-Ming.(2022).The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field-stimulated osteoblastic differentiation.FASEB JOURNAL,36(6).
MLA	He, Wen-Fang,et al."The interdependent relationship between the nitric oxide signaling pathway and primary cilia in pulse electromagnetic field-stimulated osteoblastic differentiation".FASEB JOURNAL 36.6(2022).