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Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans | |
Guo, Tao1,2; Su, Dan1; Huang, Yan1; Wang, Ya1; Li, Yong-Hui2 | |
2015-08-01 | |
发表期刊 | MOLECULES |
ISSN | 1420-3049 |
卷号 | 20期号:8页码:15273-15286 |
摘要 | In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (-)-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM) and artificial neural network (ANN). The optimal condition for ATPS with 20% n-propanol and 24% (NH4)(2)SO4 coupled with ultrasonic-assisted extraction including a solvent to solid ratio of 15:1, a temperature of 40 degrees C, and a treatment time of 55 min was obtained. Under the condition, the yield of (-)-fargesin increased 15.12%, and the purities of (-)-fargesin, sesamin and L-asarinin reached 2.222%, 1.066%, and 1.583%, with an increase of 44.38%, 25.70%, and 26.34% compared to those extracted with 95% ethanol, respectively. Coefficient of the determined (0.9855) and mean squared error (0.0018) of ANN model suggested good fitness and generalization of the ANN. Taken together, the results showed that ultrasonic-assisted ATPS can be a suitable method for extraction and enrichment of lignans from Z. armatum. |
关键词 | Zanthoxylum armatum lignans aqueous two-phase system ultrasonic-assisted extraction artificial neural network response surface methodology |
DOI | 10.3390/molecules200815273 |
收录类别 | SCIE |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS记录号 | WOS:000361375400107 |
出版者 | MDPI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/155489 |
专题 | 生命科学与工程学院 |
通讯作者 | Li, Yong-Hui |
作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 2.Hainan Med Univ, Hainan Prov Key Lab R&D Trop Plants, Haikou 571101, Peoples R China |
第一作者单位 | 生命科学与工程学院 |
第一作者的第一单位 | 生命科学与工程学院 |
推荐引用方式 GB/T 7714 | Guo, Tao,Su, Dan,Huang, Yan,et al. Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans[J]. MOLECULES,2015,20(8):15273-15286. |
APA | Guo, Tao,Su, Dan,Huang, Yan,Wang, Ya,&Li, Yong-Hui.(2015).Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans.MOLECULES,20(8),15273-15286. |
MLA | Guo, Tao,et al."Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans".MOLECULES 20.8(2015):15273-15286. |
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