Establishment and development of a CZE-UV method for rapid measurement of aprotinin potency

doi:10.1002/elps.201900387

	Establishment and development of a CZE-UV method for rapid measurement of aprotinin potency
	He, Shujuan 1; Zhang, Jing 1; Dong, Yue 1; Duan, Xiaoyun 2; Yang, Fatang 1; Luo, Tian 1; Wang, Zhen 1; Dong, Yuming 1
	2020-02-01
发表期刊	ELECTROPHORESIS
ISSN	0173-0835
卷号	41 期号:3-4 页码:168-174
摘要	A new method for the measurement of aprotinin potency by CZE-UV detector was established for the first time. The on-line mixing of substrate, trypsin and aprotinin using at-inlet technology was realized by the established method. Enzymatic reaction, separation, and detection of substrate and product can be performed simultaneously online. The aprotinin potency can be measured within 4 min. The response surface methodology was used to optimize the incubation conditions of trypsin and substrate, and the optimized conditions were obtained under 17.39 mM phosphate buffer at pH 7.6, 1.40 min of incubation time. The repeatability of proposed method was evaluated in three different systems of capillary zone electrophoresis: (i) only substrate; (ii) trypsin and substrate; (iii) aprotinin, trypsin and substrate, and the RSDs of migration times and peak areas of substrate were less than 2.7 and 3.1%, respectively. The RSDs of migration times and peak areas of product were less than 2.1 and 3.0%, respectively. A formula was also developed to calculate the aprotinin potency in this method. In a word, the established CZE-UV method was convenient, fast, and environmentally friendly for the measurement of aprotinin potency.
关键词	Aprotinin Capillary zone electrophoresis-ultraviolet detector Potency Rapid measurement
DOI	10.1002/elps.201900387
收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000497133100001
出版者	WILEY
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/155307
专题	兰州理工大学
通讯作者	Dong, Yue
作者单位	1.Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	He, Shujuan,Zhang, Jing,Dong, Yue,et al. Establishment and development of a CZE-UV method for rapid measurement of aprotinin potency[J]. ELECTROPHORESIS,2020,41(3-4):168-174.
APA	He, Shujuan.,Zhang, Jing.,Dong, Yue.,Duan, Xiaoyun.,Yang, Fatang.,...&Dong, Yuming.(2020).Establishment and development of a CZE-UV method for rapid measurement of aprotinin potency.ELECTROPHORESIS,41(3-4),168-174.
MLA	He, Shujuan,et al."Establishment and development of a CZE-UV method for rapid measurement of aprotinin potency".ELECTROPHORESIS 41.3-4(2020):168-174.