Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis

doi:10.1186/s13213-020-01563-y

	Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis
	Chen, Keyuan 1,2; Gao, Yan 1,2; Wang, Xinjian 3; Cheng, Feng 1,2; Ul Haq, Shahbaz 1,2; Liang, Yun 4; Yang, Zhiwei 4; Li, Bo 4; Liu, Yuan 4; Wang, Yonggang3
	2020-05-15
发表期刊	ANNALS OF MICROBIOLOGY
ISSN	1590-4261
卷号	70 期号:1
摘要	Purpose To investigate the effect of Danggui Buxue Tang (DBT) on intestinal microbiota diversity after fermentation by Bacillus subtilis. Methods B. subtilis was used to ferment DBT. Sprague Dawley (SD) rats were randomly divided into the following four groups with six rats in each group: the control group, DBT nonfermentation group, B. subtilis group, and DBT fermentation group. Rats were fed continuously for 14 days. The 16S rRNA of faecal samples was analysed by high-throughput Illumina sequencing. Results In total, 3483 operational taxonomical units (OTUs) were identified in this study, and 1236 OTUs were shared among all samples. Moreover, the most abundant phyla identified in this study were Bacteroidetes (29.65-38.19%) and Firmicutes (48.30-67.04%). The F/B ratios of the DBT nonfermentation group (1.07%) and the DBT fermentation group (1.78%) were slightly lower than those of the control group (2.29%). Lactobacillus was most upregulated in the DBT fermentation group (38.4%), followed by the DBT nonfermentation group (18.97%), control group (14.61%), and probiotics group (8.39%). Moreover, the pathogenic bacteria Alistipes and Parabacteroides were found to be downregulated in the DBT fermentation group (the percentages of Alistipes and Parabacteroides were as follows: control group, 8.09% and 0.16%; DBT nonfermentation group, 4.31% and 0.37%; DBT fermentation group, 1.96 and 0.09%; and probiotics group, 6.25% and 0.12%, respectively). Conclusion This study is the first to research systematically the effects of DBT on the diversity of rat intestinal microbiota before and after fermentation. The structural characteristics of complex bacterial community in each group were clearly analysed, and DBT significantly increases probiotics and inhibits pathogenic bacterial growth in the intestinal tract of rats after fermentation, which plays a significant role in maintaining the balance of the intestinal microbiota of the rats. This research provides new insights into the development and utilization of traditional Chinese medicine.
关键词	Danggui Buxue Tang Fermentation Intestinal microbiota
DOI	10.1186/s13213-020-01563-y
收录类别	SCIE
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000533076600001
出版者	SPRINGER
来源库	WOS
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/155193
专题	生命科学与工程学院
通讯作者	Hao, Baocheng
作者单位	1.Chinese Acad Agr Sci, Key Lab New Anim Drug Project Gansu Prov, Minist Agr & Rural Affairs, Lanzhou Inst Husb & Pharmaceut Sci, 335 Qilihe Rd, Lanzhou 730050, Peoples R China; 2.Chinese Acad Agr Sci, Key Lab Vet Pharmaceut Dev, Minist Agr & Rural Affairs, Lanzhou Inst Husb & Pharmaceut Sci, 335 Qilihe Rd, Lanzhou 730050, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China; 4.Pulm Hosp Lanzhou, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Chen, Keyuan,Gao, Yan,Wang, Xinjian,et al. Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis[J]. ANNALS OF MICROBIOLOGY,2020,70(1).
APA	Chen, Keyuan.,Gao, Yan.,Wang, Xinjian.,Cheng, Feng.,Ul Haq, Shahbaz.,...&Hao, Baocheng.(2020).Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis.ANNALS OF MICROBIOLOGY,70(1).
MLA	Chen, Keyuan,et al."Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis".ANNALS OF MICROBIOLOGY 70.1(2020).

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