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Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli | |
Wang, Yonggang1,2![]() ![]() ![]() | |
2020-08-04 | |
发表期刊 | PEERJ
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ISSN | 2167-8359 |
卷号 | 8 |
摘要 | The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia pastoris GS115-pPIC9K under non-induced culture conditions. Under methanol induction conditions, the growth of the GOD-transgenic strain was significantly lowered than P. pastoris GS115-pPIC9K with the induced-culture time increase, and the optical densities of GOD-transgenic strain reached one-third of that of the P. pastoris GS115-pPIC9K at 51 h. The activity of glucose oxidase in the cell-free supernatant, the supernatant of cell lysate, and the precipitation of cell lysate was 14.3 U/mL, 18.2 U/mL and 0.48 U/mL, respectively. The specific activity of glucose oxidase was 8.3 U/mg, 6.52 U/mg and 0.73 U/mg, respectively. The concentration of hydrogen peroxide formed by glucose oxidase from supernatant of the fermentation medium, the supernatant of the cell lysate, and the precipitation of cell lysate catalyzing 0.2 M glucose was 14.3 mu g/mL, 18.2 mu g/mL, 0.48 mu g/mL, respectively. The combination of different concentrations of glucose oxidase and glucose could significantly inhibit the growth of Agrobacterium and Escherichia coli in logarithmic phase. The filter article containing supernatant of the fermentation medium, supernatant of the cell lysate, and precipitation of cell lysate had no inhibitory effect on Agrobacterium and E. coli. The minimum inhibitory concentration of hydrogen peroxide on the plate culture of Agrobacterium and E. coli was 5.6 x 10(3) mu g/mL and 6.0 x 10(3) mu g/mL, respectively. |
关键词 | Aspergillus niger Pichia pastoris Glucose oxidase Transgenic Antimicrobial activity |
DOI | 10.7717/peerj.9010 |
收录类别 | SCIE |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000555317300001 |
出版者 | PEERJ INC |
来源库 | WOS |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/155124 |
专题 | 生命科学与工程学院 |
通讯作者 | Wang, Yonggang; Ma, Jianzhong |
作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China; 2.Lanzhou Univ Technol, Key Lab Drug Screening & Deep Proc Tradit Chinese, Lanzhou, Gansu, Peoples R China |
第一作者单位 | 生命科学与工程学院; 兰州理工大学 |
通讯作者单位 | 生命科学与工程学院; 兰州理工大学 |
第一作者的第一单位 | 生命科学与工程学院 |
推荐引用方式 GB/T 7714 | Wang, Yonggang,Wang, Jiangqin,Leng, Feifan,et al. Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli[J]. PEERJ,2020,8. |
APA | Wang, Yonggang,Wang, Jiangqin,Leng, Feifan,Ma, Jianzhong,&Bagadi, Alnoor.(2020).Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli.PEERJ,8. |
MLA | Wang, Yonggang,et al."Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli".PEERJ 8(2020). |
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