A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein

doi:10.1016/j.foodchem.2022.132112

	A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein
	Wang, Jinbin 1,2; Yang, Qianwen 1,3; Liu, Hua 1,2; Chen, Yifan 1,2; Jiang, Wei 1,2; Wang, Yu1,3 ; Zeng, Haijuan 1,2
	2022-06-01
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	378
摘要	Nanomaterial-based lateral flow immunoassays (LFIAs) have been widely used for the on-site detection of genetically modified components. However, the practical applications are often limited by the complex matrix, such as in red samples. In this study, a thionine (Thi) labeling-based LFIA was developed for the first time to detect CP4-EPSPS protein. The optimal labeling concentration of Thi was 0.5 mg/mL, and the antibody could be rapidly coupled to Thi in 10 min. The visual limit of detection (vLOD) levels for transgenic soybean, sugar beet, and cotton containing the CP4-EPSPS protein reached 0.05%, 0.1%, and 0.1%, respectively, and had no interference from other proteins. After storage at 4 degrees C for three months, the LFIA sensitivity remained unchanged and showed good stability. This method could be used to screen and detect a variety of transgenic crops containing the CP4-EPSPS protein, and the results were consistent with the current standard assay. This study pioneered the development of an immunochromatographic method using Thi as a marker and applied it to the detection of the CP4-EPSPS protein in herbicide-tolerant transgenic crops. This provides a new method for the rapid immunoassay of Thi as a dye and has good prospects for practical application.
关键词	Lateral flow immunoassays Thionine-labeling CP4-EPSPS protein Genetically modified crops
DOI	10.1016/j.foodchem.2022.132112
收录类别	SCIE ; EI
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000746548300010
出版者	ELSEVIER SCI LTD
EI入藏号	20220311460522
EI主题词	Proteins
EI分类号	461.9.1 Immunology ; 761 Nanotechnology ; 804.1 Organic Compounds ; 821.4 Agricultural Products ; 933.1 Crystalline Solids
来源库	WOS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/154759
专题	能源与动力工程学院
通讯作者	Zeng, Haijuan
作者单位	1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; 2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai 201106, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinbin,Yang, Qianwen,Liu, Hua,et al. A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein[J]. FOOD CHEMISTRY,2022,378.
APA	Wang, Jinbin.,Yang, Qianwen.,Liu, Hua.,Chen, Yifan.,Jiang, Wei.,...&Zeng, Haijuan.(2022).A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein.FOOD CHEMISTRY,378.
MLA	Wang, Jinbin,et al."A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein".FOOD CHEMISTRY 378(2022).