Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance

doi:10.1016/j.enzmictec.2021.109896

	Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance
	Tu, Hongyi 1,2; Zhang, Boyuan 1,2; Zhang, Xiayun 1,2; Zhao, Chunli 1,2; Li, Lin 1,2; Wang, Jianbin 1,2; Chen, Zhenbin1,2 ; Wang, Pingbo1,2 ; Li, Zhizhong 3
	2021-10
发表期刊	Enzyme and Microbial Technology
ISSN	01410229
卷号	150
摘要	A novel magnetic thermosensitive polymer composite carrier with target spacing was developed. In this strategy, thermosensitive polymer grafted on magnetic Fe3O4 for enhancing immobilized penicillin G acylase (PGA) performance and introduce immobilized target spacing into magnetic carriers for the first time. Fe3O4 nanoparticles were synthesized by a reverse microemulsion method. The modifier used was the silane coupling agent γ-methylacryloxypropyl trimethoxysilane (KH570) and then reacting with a reversible-adaptive fragmentation chain transfer (RAFT) reagent, 2-cyano-2-propyldodecyl trithiocarbonate (CPDTC). The thermo-sensitive nanoparticle-composite carrier of Fe3O4-grafted-poly N, N-diethyl acrylamide-block-poly β-Hydroxyethyl methacrylate-block-random copolymer of glycidyl methacrylate and methyl methacrylate (Fe3O4-g-PDEA-b-PHEMA-b-P(MMA-co-GMA)) were synthesized by RAFT polymerization technique that used N, N-diethyl acrylamide (DEA), β-Hydroxyethyl methacrylate (HEMA), Glycidyl methacrylate (GMA) and Methyl methacrylate (MMA) as monomer, then which were employed as functional carriers for the immobilization of PGA. Within the carrier, the epoxy group of GMA segment was a target immobilization site for PGA and the introduction of MMA reflected the target space of immobilized PGA to improve catalytic activity and catalytic activity recovery rate of the immobilized PGA. Characterizations demonstrated that the triblock copolymers grafted Fe3O4 nanoparticles were successfully fabricated by the structure design. Besides, under these circumstances the enzyme activity (EA), enzyme loading capacity (ELC) and catalytic activity recovery ration (CAR) reached 31235 U/g, 128.39 mg/g and 93.32 %, respectively. The catalytic activity of immobilized PGA maintained 87.4 % of initial value and the recovery ratio (R) of immobilized PGA reached 96.22 % after recycling 12 times. Furthermore, the immobilized PGA exhibited advantages of low temperature homogeneous catalysis and magnetic separation, which indicated broad application prospects in the biocatalysts’ field. © 2021 Elsevier Inc.
关键词	Amides Catalysis Catalyst activity Composite materials Coupling agents Drug products Enzyme activity Esters Gas metal arc welding Grafting (chemical) Iron oxides Living polymerization Magnetic separation Magnetite Microemulsions Nanoparticles PHEMA Recovery Synthesis (chemical) Temperature Adaptive fragmentation Glycidyl methacrylate Homogeneous catalysis Hydroxyethyl methacrylate Immobilized penicillin G acylase Reverse microemulsion method Silane coupling agent Thermo-sensitive polymer
DOI	10.1016/j.enzmictec.2021.109896
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000704343900007
出版者	Elsevier Inc.
EI入藏号	20213410809669
EI主题词	Acrylic monomers
EI分类号	461.9 Biology ; 538.2.1 Welding Processes ; 641.1 Thermodynamics ; 761 Nanotechnology ; 802.2 Chemical Reactions ; 802.3 Chemical Operations ; 803 Chemical Agents and Basic Industrial Chemicals ; 804 Chemical Products Generally ; 815.1 Polymeric Materials ; 815.2 Polymerization ; 933 Solid State Physics ; 951 Materials Science
来源库	WOS
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/150746
专题	材料科学与工程学院
通讯作者	Chen, Zhenbin
作者单位	1.Lanzhou Univ Technol, Coll Mat Sci & Engn, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, State Key Lab Adv Progressing & Recycling Nonferr, Lanzhou 730050, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
第一作者单位	材料科学与工程学院; 兰州理工大学
通讯作者单位	材料科学与工程学院; 兰州理工大学
第一作者的第一单位	材料科学与工程学院
推荐引用方式 GB/T 7714	Tu, Hongyi,Zhang, Boyuan,Zhang, Xiayun,et al. Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance[J]. Enzyme and Microbial Technology,2021,150.
APA	Tu, Hongyi.,Zhang, Boyuan.,Zhang, Xiayun.,Zhao, Chunli.,Li, Lin.,...&Li, Zhizhong.(2021).Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance.Enzyme and Microbial Technology,150.
MLA	Tu, Hongyi,et al."Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance".Enzyme and Microbial Technology 150(2021).