Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops

doi:10.1016/j.foodchem.2020.127627

	Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops
	Zeng, Haijuan 1; Wang, Jinbin 1; Jia, Junwei 1; Wu, Guogan 1; Yang, Qianwen 1,2; Liu, Xiaofeng2 ; Tang, Xueming 1
	2021-01-15
发表期刊	Food Chemistry
ISSN	03088146
卷号	335
摘要	A colloidal gold immunochromatographic strip (ICS) for simultaneous detection of multiple transgenic proteins, including CP4 EPSPS, BT-Cry1Ab and BT-Cry1Ac, was developed in this study. The sensitivity of the strip to the target protein was 5 ng/mL for CP4 EPSPS, 100 ng/mL for BT-Cry1Ab and Cry1Ac, respectively. Parallel analysis for maize, soybean, sugar beet and cotton showed the strip could detect 1% of transgenic content in crops containing BT-Cry1Ab and Cry1Ac, and, at least, 0.1% of content in crops containing CP4 EPSPS. The detection results for seed samples indicated the multicomponent analysis ICS had good accuracy. The analysis could be completed within 10 min and had the advantages of being high-throughput, easy to operate and visual detection. This is the first report of semi-quantitative ICS for detecting three transgenic proteins simultaneously. The developed approach may provide insights into the development of ICS for analyzing simultaneously multiple components in genetically modified crops. © 2020 Elsevier Ltd
关键词	Crops Gold nanoparticles Proteins Sugar beets Genetically modified crops Lateral flow test Multi-component analysis Multiple components Parallel analysis Simultaneous detection Target proteins Visual detection
DOI	10.1016/j.foodchem.2020.127627
收录类别	EI
语种	英语
出版者	Elsevier Ltd
EI入藏号	20203209019578
EI主题词	Chromium compounds
来源库	Compendex
分类代码	804.1 Organic Compounds - 821.4 Agricultural Products
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/115224
专题	生命科学与工程学院
作者单位	1.The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai; 201106, China; 2.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China
推荐引用方式 GB/T 7714	Zeng, Haijuan,Wang, Jinbin,Jia, Junwei,et al. Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops[J]. Food Chemistry,2021,335.
APA	Zeng, Haijuan.,Wang, Jinbin.,Jia, Junwei.,Wu, Guogan.,Yang, Qianwen.,...&Tang, Xueming.(2021).Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops.Food Chemistry,335.
MLA	Zeng, Haijuan,et al."Development of a lateral flow test strip for simultaneous detection of BT-Cry1Ab, BT-Cry1Ac and CP4 EPSPS proteins in genetically modified crops".Food Chemistry 335(2021).