Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.

doi:10.19026/ajfst.9.1665

	Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.
	Ma, Hui; Wei, Jun-Qing; Su, Dan; Guo, Tao; Feng, Zai-Ping; Zhang, Ju-Bao; Tan, Su-Bei; Wei, Li-Yang
	2015
发表期刊	Advance Journal of Food Science and Technology
ISSN	20424868
卷号	9 期号:10 页码:807-811
摘要	Aqueous two-phase system is a useful method for purifying bio-logical materials in biochemistry and biotechnology. In this study the potential use of a K2HPO4/ethanol aqueous two-phase system to extract polysaccharides from Potentilla anserine L. The effects of salt, ethanol, NaCl concentrate and pH on the partitioning of polysaccharides were studied. The ATPS with 27% ethanol, 21% K2HPO4, 0.4 mol/L NaCl and pH 7 were selected as the optimal process system according to the partition coefficient and recovery. Furthermore, a comparison between the ATPS and the conventional extraction was conducted. For ATPS, the extraction yield and purity were 12.47 and 16.18%; while the extraction yield and purity were 10.3 and 6.14% by conventional extraction, respectively. ATPS showed a higher the extraction yield and the purity was around 2-fold with conventional method. So the ATPS can be employed to become an efficient and prospective process for the extraction. © 2015 Maxwell Scientific Publication Corp.
关键词	Ethanol Polysaccharides Solvent extraction Aqueous two phase system Aqueous two-phase extraction Conventional extraction Conventional methods Extraction yield Optimal process Partition coefficient Potentilla anserina
DOI	10.19026/ajfst.9.1665
收录类别	EI
语种	英语
出版者	Maxwell Science Publications
EI入藏号	20160801986764
EI主题词	Extraction
EI分类号	802.3 Chemical Operations - 804.1 Organic Compounds
来源库	Compendex
分类代码	802.3 Chemical Operations - 804.1 Organic Compounds
引用统计	无
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/112328
专题	兰州理工大学
作者单位	Department of Gansu Education, The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China
第一作者单位	生命科学与工程学院
第一作者的第一单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Ma, Hui,Wei, Jun-Qing,Su, Dan,et al. Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.[J]. Advance Journal of Food Science and Technology,2015,9(10):807-811.
APA	Ma, Hui.,Wei, Jun-Qing.,Su, Dan.,Guo, Tao.,Feng, Zai-Ping.,...&Wei, Li-Yang.(2015).Aqueous two-phase extraction of polysaccharide from Potentilla anserine L..Advance Journal of Food Science and Technology,9(10),807-811.
MLA	Ma, Hui,et al."Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.".Advance Journal of Food Science and Technology 9.10(2015):807-811.