Lanzhou University of Technology Institutional Repository （LUT_IR）
| Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium | |
Xie, Yan-Fang1; Shi, Wen-Gui2; Zhou, Jian1; Gao, Yu-Hai1; Li, Shao-Feng1; Fang, Qing-Qing1; Wang, Ming-Gang3 ; Ma, Hui-Ping4; Wang, Ju-Fang2; Xian, Cory J.5
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| 2016-12 | |
| 发表期刊 | BONE
 |
| ISSN | 8756-3282 |
| 卷号 | 93页码:22-32 |
| 摘要 | Pulsed electromagnetic fields (PEMFs) have been considered as a potential candidate for the prevention and treatment of osteoporosis, however, the mechanism of its action is still elusive. We have previously reported that 50 Hz 0.6 mT PEMF5 stimulate osteoblastic differentiation and mineralization in a primary cilium-dependent manner, but did not know the reason. In the current study, we found that the PEMFs promoted osteogenic differentiation and maturation of rat calvarial osteoblasts (ROBs) by activating bone morphogenetic protein BMPSmad1/5/8 signaling on the condition that primary cilia were normal. Further studies revealed that BMPRII, the primary binding receptor of BMP ligand, was readily and strongly upregulated by PEMF treatment and localized at the bases of primary cilia. Abrogation of primary cilia with small interfering RNA sequence targeting IFT88 abolished the PEMF-induced upregulation of BMPRII and its ciliary localization. Knockdown of BMPRII expression level with RNA interference had no effects on primary cilia but significantly decreased the promoting effect of PEMF5 on osteoblastic differentiation and maturation. These results indicated that PEMFs stimulate osteogenic differentiation and maturation of osteoblast by primary cilium-mediated upregulation of BMPRII expression and subsequently activation of BMP-Smad1/5/8 signaling, and that BMPRII is the key component linking primary cilium and BMP-Smad1/5/8 pathway. This study has thus revealed the molecular mechanism for the osteogenic effect of PEMFs. (C) 2016 Elsevier Inc. All rights reserved. |
| 关键词 | Pulsed electromagnetic fields Osteoblasts Primary cilia BMP-Smad1/5/8 signaling BMPRII |
| DOI | 10.1016/j.bone.2016.09.008 |
| 收录类别 | SCI ; SCIE |
| 语种 | 英语 |
| 资助项目 | NHMRC Australia Senior Research Fellowship[1042105] |
| WOS研究方向 | Endocrinology & Metabolism |
| WOS类目 | Endocrinology & Metabolism |
| WOS记录号 | WOS:000386987500003 |
| 出版者 | ELSEVIER SCIENCE INC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/33534 |
| 专题 | 高等教育研究所（教学质量监督与评估工作办公室、本科教学督导委员会研究生督导委员会） |
| 通讯作者 | Chen, Ke-Ming |
| 作者单位 | 1.CPLA, Lanzhou Command, Lanzhou Gen Hosp, Inst Orthopaed, Lanzhou 730050, Peoples R China; 2.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 4.CPLA, Lanzhou Command, Lanzhou Gen Hosp, Dept Pharm, Lanzhou 730050, Peoples R China; 5.Univ South Australia, Sansom Inst Hlth Res, Sch Pharm & Med Sci, Adelaide, SA 5001, Australia |
| 推荐引用方式 GB/T 7714 | Xie, Yan-Fang,Shi, Wen-Gui,Zhou, Jian,et al. Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium[J]. BONE,2016,93:22-32. |
| APA | Xie, Yan-Fang.,Shi, Wen-Gui.,Zhou, Jian.,Gao, Yu-Hai.,Li, Shao-Feng.,...&Chen, Ke-Ming.(2016).Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium.BONE,93,22-32. |
| MLA | Xie, Yan-Fang,et al."Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium".BONE 93(2016):22-32. |
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