Fluorescent glutathione probe based on MnO2-Si quantum dots nanocomposite directly used for intracellular glutathione imaging
Ma, Hong1,2; Li, Xinran1,2; Liu, Xiaoyu1,2,3; Deng, Min1,2; Wang, Xudong1,2; Iqbal, Anam1,2; Liu, Weisheng1,2; Qin, Wenwu1,2
2018-02
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号255页码:1687-1693
摘要A highly sensitive glutathione probe based on silicon quantum dots (SiQDs) modified with MnO2 nanosheets has been developed. A strong reduction of the blue fluorescence of the SiQDs happened due to the surface energy transfer (SET) from SiQDs to the deposited MnO2. Fluorescence can be restored in the presence of glutathione on account of the MnO2 nanosheets are reduced. A fluorometric glutathione detection assay with a low detection limit (153 nM, at an S/N ratio of 3) can be designed via this method. It can be applied to directly determine GSH in living cells. A significant blue fluorescence image is produced in the cytosol of the cell and the fluorescence disappears while the cells treated with scavenger of GSH. (C) 2017 Elsevier B.V. All rights reserved.
关键词Silicon quantum dots Fluorescent probe Glutathione Cell culture Intracellular imaging
DOI10.1016/j.snb.2017.08.170
收录类别SCI
语种英语
资助项目Ministry of Science and Technology of China[2014DFA31890] ; National Natural Science Foundation of China[21771092]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000414319900064
出版者ELSEVIER SCIENCE SA
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://ir.lut.edu.cn/handle/2XXMBERH/32804
专题石油化工学院
通讯作者Qin, Wenwu
作者单位1.Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China
2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
3.Lanzhou Univ Technol, Coll Petrochem Technol, Lanzhou 730050, Gansu, Peoples R China
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GB/T 7714
Ma, Hong,Li, Xinran,Liu, Xiaoyu,et al. Fluorescent glutathione probe based on MnO2-Si quantum dots nanocomposite directly used for intracellular glutathione imaging[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:1687-1693.
APA Ma, Hong.,Li, Xinran.,Liu, Xiaoyu.,Deng, Min.,Wang, Xudong.,...&Qin, Wenwu.(2018).Fluorescent glutathione probe based on MnO2-Si quantum dots nanocomposite directly used for intracellular glutathione imaging.SENSORS AND ACTUATORS B-CHEMICAL,255,1687-1693.
MLA Ma, Hong,et al."Fluorescent glutathione probe based on MnO2-Si quantum dots nanocomposite directly used for intracellular glutathione imaging".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):1687-1693.
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