Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum

doi:10.1007/s10570-019-02712-6

	Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum
	Ren, Haiwei1,2 ; Shen, Jiali 1; Pei, Jiawen 1; Wang, Zhiye 2; Peng, Zhangpu 2; Fu, Shanfei 3; Zheng, Yi 4
	2019-10
发表期刊	CELLULOSE
ISSN	0969-0239
卷号	26 期号:15 页码:8367-8381
摘要	Microcrystalline cellulose (MCC) has been widely used in medicine, food and cosmetic industries. In this study, a combination method by using hydrochloric acid hydrolysis and fibrolytic enzyme purification was studied to extract MCC from sweet sorghum. The response surface methodology was employed to optimize the hydrochloric acid hydrolysis condition and therefore to maximize the MCC yield and cellulose content (purity). The optimal conditions for the hydrochloric acid hydrolysis were determined to be acid concentration of 7.0%, liquid-solid ratio of 17.3:1, time of 90 min, and temperature of 40 degrees C. Under such conditions, the yield and cellulose content of acid-extracted MCC were 81.8% and 93.2%, respectively. For enzyme refining of acid-extracted MCC, the optimum conditions were enzyme dosage of 4000 U/g substrate and time of 2 h, with which the yield, cellulose content and DP of the refined MCC were 80.03%, 99.80% and 287, respectively which were comparable to that of the commercial MCC (Lowa (R) PH101). Scanning electron microscopy, X-ray diffraction, Fourier transmission infrared spectroscopy, thermogravimetric analysis (TGA), and C-13 NMR were used to characterize the refined MCC. The refined MCC demonstrated rod-shaped morphologies, and had a series of characteristic absorption peaks and chemical groups pertain to cellulose as similar to the Lowa (R) PH101. The X-ray diffraction pattern and C-13 NMR spectrum reflected that the refined MCC had typical cellulose I structure. TGA indicated that the refined MCC had good thermal stability. This study showed sweet sorghum is a potential low-cost raw material for MCC production, and the combined acid-enzyme extraction method is promising to extract high purity MCC from cellulosic substrate.
关键词	Sweet sorghum Microcrystalline cellulose Response surface methodology Structural characterization
DOI	10.1007/s10570-019-02712-6
收录类别	SCI ; CPCI
语种	英语
资助项目	Hong Liu Excellent Young Cultivation Project of Lanzhou University of Technology[YQ2018]
WOS研究方向	Materials Science ; Polymer Science
WOS类目	Materials Science, Paper & Wood ; Materials Science, Textiles ; Polymer Science
WOS记录号	WOS:000489757400013
出版者	SPRINGER
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/31553
专题	生命科学与工程学院
通讯作者	Fu, Shanfei
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd, Lanzhou 730050, Gansu, Peoples R China 2.Gansu Acad Sci, Inst Biol, South Dingxi Rd, Lanzhou 730000, Gansu, Peoples R China 3.Jiangnan Univ, Sch Environm & Civil Engn, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China 4.Kansas State Univ, Dept Grain Sci & Ind, 101C BIVAP,1980 Kimball Ave, Manhattan, KS 66506 USA
第一作者单位	生命科学与工程学院
第一作者的第一单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Ren, Haiwei,Shen, Jiali,Pei, Jiawen,et al. Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum[J]. CELLULOSE,2019,26(15):8367-8381.
APA	Ren, Haiwei.,Shen, Jiali.,Pei, Jiawen.,Wang, Zhiye.,Peng, Zhangpu.,...&Zheng, Yi.(2019).Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum.CELLULOSE,26(15),8367-8381.
MLA	Ren, Haiwei,et al."Characteristic microcrystalline cellulose extracted by combined acid and enzyme hydrolysis of sweet sorghum".CELLULOSE 26.15(2019):8367-8381.

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