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Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids | |
Ke, Jing1; Duan, Xiao-Yun2; Liu, Xiao-Feng2; Dong, Yu-Ming1 | |
2019-10 | |
发表期刊 | CHEMICAL PAPERS |
ISSN | 2585-7290 |
卷号 | 73期号:10页码:2417-2426 |
摘要 | In this work, an isocratic micellar chromatographic method for separation of 17 free amino acids was first established. Mixed anionic and non-ionic surfactants were used as mobile phase additives. Besides, a chromatographic environment was simulated and the possible mechanism was discussed. Pre-column derivatization with 9-fluorenylmethyl chloroformate was used before chromatographic analysis. The optimized chromatographic conditions were the mobile phase A (0.075M sodium dodecyl sulfate solutions and 0.010M polyoxyethylene lauryl ether containing 20mM ammonium acetate at pH 3.5) and B (100% acetonitrile) (85:15, v/v) running at 1.2mL/min by a Venusil XBPC18 column (5 mu m, 250x4.6mm) at 35.0 degrees C. Compared with the conventional reversed phase liquid chromatography, the consumption of organic solvent in the method is lower which reduces the analysis cost and being environment-friendly. In addition, complex gradient elution is not required. This provides an alternative way for the separation of amino acids. |
关键词 | Amino acids Micellar liquid chromatography Mixed surfactants Polyoxyethylene lauryl ether Sodium dodecyl sulfate |
DOI | 10.1007/s11696-019-00791-0 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000474390300005 |
出版者 | SPRINGER INTERNATIONAL PUBLISHING AG |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/31529 |
专题 | 生命科学与工程学院 |
作者单位 | 1.Lanzhou Univ, Sch Pharm, Inst Pharmaceut Anal, Lanzhou 730000, Gansu, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Ke, Jing,Duan, Xiao-Yun,Liu, Xiao-Feng,et al. Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids[J]. CHEMICAL PAPERS,2019,73(10):2417-2426. |
APA | Ke, Jing,Duan, Xiao-Yun,Liu, Xiao-Feng,&Dong, Yu-Ming.(2019).Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids.CHEMICAL PAPERS,73(10),2417-2426. |
MLA | Ke, Jing,et al."Isocratic micellar liquid chromatography using mixed anionic and non-ionic surfactants as mobile phase additives for separation of 17 free amino acids".CHEMICAL PAPERS 73.10(2019):2417-2426. |
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