Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling

doi:10.1016/j.jenvman.2024.120327

	Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling
	Ren, Haiwei1,2 ; Li, Jinlian 1,2; Lan, Yuanyuan 1; Lu, Nana 1,2; Tian, Hui 1; Li, Jinping1,2 ; Zhang, Zhiping 3; Li, Lianhua 4; Sun, Yongming 4; Zheng, Yi 5
	2024-03
发表期刊	Journal of Environmental Management
ISSN	0301-4797
卷号	354
摘要	Sweet sorghum, as a seasonal energy crop, is rich in cellulose and hemicellulose that can be converted into biofuels. This work aims at investigating the effects of synergistic regulation of Pichia anomala and cellulase on ensiling quality and microbial community of sweet sorghum silages as a storage and pretreatment method. Furthermore, the combined pretreatment effects of ensiling and ball milling on sweet sorghum were evaluated by microstructure change and enzymatic hydrolysis. Based on membership function analysis, the combination of P. anomala and cellulase (PA + CE) significantly improved the silage quality by preserving organic components and promoting fermentation characteristics. The bioaugmented ensiling with PA + CE restructured the bacterial community by facilitating Lactobacillus and inhibiting undesired microorganisms by killer activity of P. anomala. The combined bioaugmented ensiling pretreatment with ball milling significantly increased the enzymatic hydrolysis efficiency (EHE) to 71%, accompanied by the increased specific surface area and decreased pore size/crystallinity of sweet sorghum. Moreover, the EHE after combined pretreatment was increased by 1.37 times compared with raw material. Hence, the combined pretreatment was demonstrated as a novel strategy to effectively enhance enzymatic hydrolysis of sweet sorghum. © 2024 Elsevier Ltd
关键词	Cellulose Crops Enzymatic hydrolysis Microorganisms Milling (machining) Pore size Quality control Saccharification Ball milling pretreatment Bioaugmented ensiling Cellulase Cellulose and hemicellulose Combined pre treatments Energy crops Enzymatic saccharification Pichia anomala Pre-treatments Sweet sorghum
DOI	10.1016/j.jenvman.2024.120327
收录类别	EI ; SCIE
语种	英语
资助项目	Key Project of Natural Science Foundation of Gansu Province [21JR7RA203]; National Natural Science Foundation of China [51666010]; Gansu Provincial Higher Education Industry Support Project [2023CYZC-23]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:001185176600001
出版者	Academic Press
EI入藏号	20240815578972
EI主题词	Ball milling
EI分类号	461.9 Biology ; 604.2 Machining Operations ; 801.2 Biochemistry ; 802.2 Chemical Reactions ; 802.3 Chemical Operations ; 804.1 Organic Compounds ; 811.3 Cellulose, Lignin and Derivatives ; 815.1.1 Organic Polymers ; 821.4 Agricultural Products ; 913.3 Quality Assurance and Control ; 931.2 Physical Properties of Gases, Liquids and Solids ; 951 Materials Science
原始文献类型	Journal article (JA)
EISSN	1095-8630
引用统计	无
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/169942
专题	生命科学与工程学院能源与动力工程学院
通讯作者	Zheng, Yi
作者单位	1.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou; 730050, China; 2.Key Laboratory of Complementary Energy System of Biomass and Solar Energy, Lanzhou University of Technology, 730050, China; 3.Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Henan Agricultural University, Zhengzhou; 450002, China; 4.Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou; 510640, China; 5.Department of Grain Science and Industry, Kansas State University, 101C BIVAP, 1980 Kimball Avenue, Manhattan; KS; 66506, United States
第一作者单位	生命科学与工程学院; 兰州理工大学
第一作者的第一单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Ren, Haiwei,Li, Jinlian,Lan, Yuanyuan,et al. Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling[J]. Journal of Environmental Management,2024,354.
APA	Ren, Haiwei.,Li, Jinlian.,Lan, Yuanyuan.,Lu, Nana.,Tian, Hui.,...&Zheng, Yi.(2024).Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling.Journal of Environmental Management,354.
MLA	Ren, Haiwei,et al."Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling".Journal of Environmental Management 354(2024).