Evaluating the antioxidant activity of secondary metabolites of endophytic fungi from Hypericum perforatum L. by an electrochemical biosensor based on AuNPs/AC@CS composite
Bolu Sun; Yanmei Yang; Yanlei Sun; Dan Wu; Lei Kan; Chengyang Gao; Hongxia Shi; Chunyan Sang; Tiankun Zhao; Lin Yang
2023
发表期刊Bioelectrochemistry
ISSN1567-5394
卷号151页码:108400
摘要

Due to the variety and activity of secondary metabolites of endophytic fungi (SMEF) from medicinal plants, and the operation cumbersome of existing methods for evaluating the activity, there is urgent to establish a simple, efficient and sensitive evaluation and screening technology. In this study, the prepared chitosan functionalized activated carbon (AC@CS) composite as the electrode substrate material was used to modify glassy carbon electrode (GCE), and the gold nanoparticles (AuNPs) was deposited on AC@CS/GCE by cyclic voltammetry (CV). A ds-DNA/AuNPs/AC@CS/GCE electrochemical biosensor for evaluating the antioxidant activity of SMEF from Hypericum perforatum L. (HP L.) was fabricated using the method of layer by layer assembly. The experimental conditions affecting the evaluation results of the biosensor were optimized by square wave voltammetry (SWV) using Ru(NH3)3+ 6 as the probe, and the antioxidant activity of various SMEF from HP L. was evaluated by the proposed biosensor. Meanwhile, the results of the biosensor were also verified by UV-Vis. According to the optimized experimental results, the biosensors had a high levels of oxidative DNA damage at pH 6.0 and Fenton solution system with Fe2+ to OH- ratio of 1:3 for 30 min. Among the crude extracts of SMEF from roots, stems and leaves of HP L., the crude extracts from stems presents a high antioxidant activity, but it was weaker than L-ascorbic acid. This result was consistent with the evaluation results of UV-Vis spectrophotometric method, also the fabricated biosensor presents high stability and sensitivity. This study not only provides a novel, convenient and efficient way for rapid evaluating the antioxidant activity of a wide variety of SMEF from HP L., but also provides a novel evaluation strategy for the SMEF from medicinal plants.

关键词Hypericum perforatum L. (HP L.) Secondary metabolites of endophytic fungi (SMEF) Electrochemical biosensor Evaluation of antioxidant activity
DOIhttps://doi.org/10.1016/j.bioelechem.2023.108400
收录类别EI ; SCIE
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry
WOS类目Biochemistry & Molecular Biology ; Biology ; Biophysics ; Electrochemistry
WOS记录号WOS:000944506900001
出版者Elsevier B.V.
EI入藏号20230913632804
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://ir.lut.edu.cn/handle/2XXMBERH/161037
专题生命科学与工程学院
作者单位1.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
2.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
3.Department of Endocrinology, Wuhan Third Hospital, China, 430000.
4.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
5.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
6.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
7.Lanzhou zhongjianke Testing Technology Co., Ltd, Lanzhou 730000, Gansu.
8.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, People’s Republic of China
9.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
10.School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou 730050, Gansu, China.
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GB/T 7714
Bolu Sun,Yanmei Yang,Yanlei Sun,et al. Evaluating the antioxidant activity of secondary metabolites of endophytic fungi from Hypericum perforatum L. by an electrochemical biosensor based on AuNPs/AC@CS composite[J]. Bioelectrochemistry,2023,151:108400.
APA Bolu Sun.,Yanmei Yang.,Yanlei Sun.,Dan Wu.,Lei Kan.,...&Xiaofeng shi.(2023).Evaluating the antioxidant activity of secondary metabolites of endophytic fungi from Hypericum perforatum L. by an electrochemical biosensor based on AuNPs/AC@CS composite.Bioelectrochemistry,151,108400.
MLA Bolu Sun,et al."Evaluating the antioxidant activity of secondary metabolites of endophytic fungi from Hypericum perforatum L. by an electrochemical biosensor based on AuNPs/AC@CS composite".Bioelectrochemistry 151(2023):108400.
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