Sandwich-likely structured, magnetically-driven recovery, biomimetic composite penicillin G acylase-based biocatalyst with excellent operation stability
Liu, Chunli1,2; Zhou, Yongshan1,2; Wu, Gang1,2; Gao, Kaikai1,2; Li, Lin1,2; Tu, Hongyi1,2; Chen, Zhenbin1,2
2022-04-20
发表期刊Colloids and Surfaces A: Physicochemical and Engineering Aspects
ISSN0927-7757
卷号639
摘要Immobilization of penicillin G acylase (PGA) on carrier was an established approach to improve the stability and reusable property of it. Herein, Magnetic Fe3O4 nanoparticles (Fe3O4 NPs) that coated by poly (tannic acid), named as Fe3O4 @PTA nanoparticles (Fe3O4 @PTA NPs), had been used as carrier for immobilizing of PGA by physical adsorption effect to produce Fe3O4 @PTA-PGA NPs. Then Fe3O4 @PTA-PGA NPs was post-modified by spontaneous in-situ self-polymerization of dopamine to prepare polydopamine (PDA) coated Fe3O4 @PTA-PGA/PDA nanocapsule. Fe3O4 @PTA-PGA/PDA nanocapsule with sandwich-like structure was a biomimetic composite penicillin G acylase-based biocatalyst, having excellent operational stability. The response rate of Fe3O4 @PTA-PGA/PDA nanocapsule decreased with the extension of polymerization time, whereas the storage stability, thermal stability and pH stability increased with the extension of polymerization time. The EAR of Fe3O4 @PTA-PGA/PDA nanocapsule was 76.4 % with PDA coating time of 4 h. The relative activity (Rt) of Fe3O4 @PTA-PGA/PDA magnetic nanocapsule was 87.1 %, while that of free PGA was 12.5 % after 90 days of storage. Moreover, the Fe3O4 @PTA-PGA/PDA nanocapsule still had 94.1 % of its initial enzyme activity retained and with a enzyme recovery ratio (Re) of 98.0 % after 12 cycles of repeated usage. All those results indicated that the Fe3O4 @PTA-PGA/PDA nanocapsule had excellent operation stability and reusable property, which was very promising for being used as biocatalyst in industrial applications. © 2022
关键词Amines Drug products Enzyme activity Enzyme immobilization Nanocapsules Nanomagnetics pH effects Polymerization Stability Biocatalyst Biomimetic composites Immobilisation Magnetic nanocapsules Operation stability Operational stability Penicillin G Acylase Polydopamine Polymerization time Property
DOI10.1016/j.colsurfa.2021.128245
收录类别EI ; SCIE
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Physical
WOS记录号WOS:000770656600003
出版者Elsevier B.V.
EI入藏号20220411508597
EI主题词Magnetite
EI分类号461.9 Biology ; 701.2 Magnetism: Basic Concepts and Phenomena ; 761 Nanotechnology ; 801.1 Chemistry, General ; 804.1 Organic Compounds ; 815.2 Polymerization ; 933 Solid State Physics
来源库WOS
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://ir.lut.edu.cn/handle/2XXMBERH/158039
专题材料科学与工程学院
理学院
通讯作者Chen, Zhenbin
作者单位1.Lanzhou Univ Technol, Coll Mat Sci & Engn, Lanzhou 730050, Peoples R China;
2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Peoples R China
第一作者单位材料科学与工程学院;  省部共建有色金属先进加工与再利用国家重点实验室
通讯作者单位材料科学与工程学院;  省部共建有色金属先进加工与再利用国家重点实验室
第一作者的第一单位材料科学与工程学院
推荐引用方式
GB/T 7714
Liu, Chunli,Zhou, Yongshan,Wu, Gang,et al. Sandwich-likely structured, magnetically-driven recovery, biomimetic composite penicillin G acylase-based biocatalyst with excellent operation stability[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects,2022,639.
APA Liu, Chunli.,Zhou, Yongshan.,Wu, Gang.,Gao, Kaikai.,Li, Lin.,...&Chen, Zhenbin.(2022).Sandwich-likely structured, magnetically-driven recovery, biomimetic composite penicillin G acylase-based biocatalyst with excellent operation stability.Colloids and Surfaces A: Physicochemical and Engineering Aspects,639.
MLA Liu, Chunli,et al."Sandwich-likely structured, magnetically-driven recovery, biomimetic composite penicillin G acylase-based biocatalyst with excellent operation stability".Colloids and Surfaces A: Physicochemical and Engineering Aspects 639(2022).
条目包含的文件
条目无相关文件。
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Liu, Chunli]的文章
[Zhou, Yongshan]的文章
[Wu, Gang]的文章
百度学术
百度学术中相似的文章
[Liu, Chunli]的文章
[Zhou, Yongshan]的文章
[Wu, Gang]的文章
必应学术
必应学术中相似的文章
[Liu, Chunli]的文章
[Zhou, Yongshan]的文章
[Wu, Gang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。