Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid

doi:10.1016/j.fitote.2021.105021

	Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid
	Si, Yanpo 1; Li, Xiaofei 1; Guo, Tao 1; Wei, Wenjun ; Zhang, Jubao 2; Jia, An ; Wang, Ya2,3 ; Zhao, Aihong 2; Chang, Jun 4; Feng, Shuying 5
	2021-10
发表期刊	FITOTERAPIA
ISSN	0367-326X
卷号	154
摘要	Bark of Phellodendron chinense Schneid. (Rutaceae), called Huang Bai in China, is one of the 50 most used Chinese medicines in clinical practice. In this paper, a new isoquinoline alkaloid glycoside was isolated from P. chinense, and its structure was elucidated using spectroscopic method. The compound was eventually identified as (1S, 3 '' S)-1, 2, 3, 4-tetrahydro-7-hydroxy-1-[(4-hydroxybenzyl) methyl]-2-methyl-8-O-isoquinolinyl-[3-hydroxy-3-methylglutaryl]-beta-D-glucopyranoside and named as Phellodendronoside A (PDA). The results of molecular docking showed that PDA could stably bind to an extracellular signal-regulated kinase (ERK), stress-activated protein kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK) proteins that are closely related to inflammation. Further, the anti-inflammatory activity of PDA was evaluated using the lipopolysaccharide (LPS) induced RAW264.7 macrophage model. We observed that PDA can effectively reduce the levels of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and decrease the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, we found that PDA inhibits the activation of ERK, JNK and p38MAPK proteins in the MAPK signaling pathway. Collectively, the present study demonstrates that PDA has excellent anti-inflammatory effect in vitro by inhibiting the overproduction of pro-inflammatory mediators, and its mechanism of action involves suppressing the activation of MAPK pathways, suggesting that PDA may be a potential agent for the treatment of inflammatory illness.
关键词	Phellodendron chinense Phellodendronoside A Anti-inflammatory MAPK pathway Molecular docking
DOI	10.1016/j.fitote.2021.105021
收录类别	SCIE
语种	英语
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000703820600010
出版者	ELSEVIER
来源库	WOS
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/149957
专题	生命科学与工程学院
通讯作者	Guo, Tao; Feng, Shuying
作者单位	1.Henan Univ Chinese Med, Dept Pharm, Zhengzhou 450046, Peoples R China; 2.Lanzhou Univ technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 3.Huanghe Sci & Technol Coll, Sch Med, Zhengzhou 450063, Peoples R China; 4.Fudan Univ, Human Phenome Inst, Shanghai 201203, Peoples R China; 5.Henan Univ Chinese Med, Coll Med, Zhengzhou 450046, Peoples R China
推荐引用方式 GB/T 7714	Si, Yanpo,Li, Xiaofei,Guo, Tao,et al. Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid[J]. FITOTERAPIA,2021,154.
APA	Si, Yanpo.,Li, Xiaofei.,Guo, Tao.,Wei, Wenjun.,Zhang, Jubao.,...&Feng, Shuying.(2021).Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid.FITOTERAPIA,154.
MLA	Si, Yanpo,et al."Isolation and characterization of phellodendronoside A, a new isoquinoline alkaloid glycoside with anti-inflammatory activity from Phellodendron chinense Schneid".FITOTERAPIA 154(2021).