A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops

doi:10.1016/j.foodchem.2021.129901

	A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops
	Zeng, Haijuan 1,2; Yang, Qianwen 1,3; Liu, Hua 1,2; Wu, Guogan 1,2; Jiang, Wei 1,2; Liu, Xiaofeng3 ; Wang, Jinbin 1,2; Tang, Xueming 1,2,4
	2021-11-01
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	361
摘要	A simple electrochemical immunosensor based on nitrogen-doped graphene and polyamide-amine (GN-PAM) composites was proposed for the detection of the CP4-EPSPS protein in genetically modified (GM) crops. In this immunosensor, the amplification of the detection signal was realized through antibodies labeled with gold nanoparticles (AuNPs). The electrochemical responses of the immunosensor were linear (R-2 = 0.9935 and 0.9912) when the GM soybean RRS and maize NK603 content ranged from 0.025% to 1.0% and 0.05% to 1.5%, respectively. The limits of detection for the GM soybean RRS and maize NK603 were as low as 0.01% and 0.03%, respectively. The immunosensor also exhibited high specificity, and satisfactory stability, reproducibility, and accuracy. Our findings indicated that the constructed immunosensor provides a new approach for the sensitive detection of the CP4-EPSPS protein. Notably, the sensor may be applied to other proteins or pathogenic bacteria by simply changing the antibodies, and may also be used for multi-component analysis.
关键词	Immunosensor Gold nanoparticle-antibody Graphene and PAMAM composites Genetically modified crops
DOI	10.1016/j.foodchem.2021.129901
收录类别	EI ; SCOPUS ; SCIE
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000664737400003
出版者	ELSEVIER SCI LTD
EI入藏号	20212310451822
EI主题词	Antibodies
EI分类号	461.9.1 Immunology - 741.1.2 Fiber Optics - 761 Nanotechnology - 801 Chemistry - 804 Chemical Products Generally - 821.4 Agricultural Products
来源库	WOS
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/148999
专题	生命科学与工程学院
通讯作者	Wang, Jinbin; Tang, Xueming
作者单位	1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; 2.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai 201106, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Haijuan,Yang, Qianwen,Liu, Hua,et al. A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops[J]. FOOD CHEMISTRY,2021,361.
APA	Zeng, Haijuan.,Yang, Qianwen.,Liu, Hua.,Wu, Guogan.,Jiang, Wei.,...&Tang, Xueming.(2021).A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops.FOOD CHEMISTRY,361.
MLA	Zeng, Haijuan,et al."A sensitive immunosensor based on graphene-PAMAM composites for rapid detection of the CP4-EPSPS protein in genetically modified crops".FOOD CHEMISTRY 361(2021).