Bioassay-guided Isolation and Identification of a acetylcholinesterase inhibitors from Calophyllum polyanthum

doi:10.4028/www.scientific.net/AMR.634-638.1225

	Bioassay-guided Isolation and Identification of a acetylcholinesterase inhibitors from Calophyllum polyanthum
	Zhang, Fei; Ren, Hai-Wei; Wang, Yong-Gang; Wang, Rong; Sheng, Jie
	2013
会议名称	2012 2nd International Conference on Chemical, Material and Metallurgical Engineering, ICCMME 2012
会议录名称	Advanced Materials Research
卷号	634-638
期号	1
页码	1225-1228
会议日期	December 15, 2012 - December 16, 2012
会议地点	Kunming, China
出版者	Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany
摘要	A significant acetylcholinesterase inhibitory activity was observed for the Ethanolic extract from the leaves of Calophyllum polyanthum by using TLC bioautographic method. Further bioassay-guided isolation of this extract using TLC bioautographic method resulted in obtaining a pyranochromanone, apetalic acid (1). The structure of 1 was identified by comparison of it's spectral characteristics with previous reports. The concentration required for 50% inhibition of 1 was 0.95 mM, determined by a microplate assay. The anti-acetylcholinesterase aity of compound 1 was weak, but it was the first pyranochromanone which have anti-acetyl cholinesterase activity. As a new leading compound, it can be modified and transformed to obtain more potently active compounds. © (2013) Trans Tech Publications, Switzerland.
关键词	Metallurgical engineering Acetylcholinesterase Active compounds Calophyllum polyanthum Cholinesterase activity Ethanolic extracts Inhibitory activity Isolation and identification Microplate assay Pyranochromanone Spectral characteristics TLC bioautographic method
DOI	10.4028/www.scientific.net/AMR.634-638.1225
收录类别	EI
语种	英语
EI入藏号	20130616000666
EI主题词	Bioassay
ISSN	10226680
来源库	Compendex
分类代码	531 Metallurgy and Metallography - 801 Chemistry
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	会议论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/117575
专题	生命科学与工程学院科学技术处（军民融合领导小组办公室）学科建设与学位办公室党委组织部（党校、帮扶办）
作者单位	School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China
第一作者单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Zhang, Fei,Ren, Hai-Wei,Wang, Yong-Gang,et al. Bioassay-guided Isolation and Identification of a acetylcholinesterase inhibitors from Calophyllum polyanthum[C]:Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany,2013:1225-1228.