Saccharification for distiller's grains based on combined hydrolysis with acid and enzyme

doi:10.3969/j.issn.1002-6819.2013.05.032

	Saccharification for distiller's grains based on combined hydrolysis with acid and enzyme
	Ren, Haiwei1,2 ; Li, Jinping2 ; Zhang, Yi 1; Li, Zhizhong 1; Yin, Jianbo3 ; Wang, Xiaomei3 ; Shen, Yongqian3 ; Shi, Jincai 4
	2013-03-01
发表期刊	Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering
ISSN	10026819
卷号	29 期号:5 页码:243-250
摘要	China is a big country of white spirit production and consumption. When brewing white spirit, large amounts of distiller's grains (DG) are left over, which are as the byproduct in white spirit production. In virtue of high content of cellulose and hemicellulose, DG is a kind of lignocellulosic biomass. In order to make full use of a distiller's grains (DG), the feasibility of using DG in making fermentable sugars by the two-step hydrolysis method of mixed acid and cellulase was explored. The key parameters of temperature, mixed acid concentrations, solid-liquid ratio and hydrolysis time were studied with the index of the concentrations of reducing sugar and xylose. In addition, the structural characteristics of DG in different hydrolysis stages were examined by means of scanning electronic microscopy (SEM), infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results indicated that there was 59.32 g/L reducing sugar and 6.49 g/L xylose in the conditions of temperature 100°C, solid-liquid ratio 1:12 g·mL-1, acid concentrations 2.0% and time 120 min, with the conversions of 77.38% and 62.50% for hemicellulose and cellulose, respectively. There were 13.27 g/L reducing sugars in the enzymatic hydrolysis conditions of cellulase dosage 4000 U/g, temperature 45°C, pH value 4.8 and time 2.5 h. The conversion rate and enzymatic hydrolyzation of the cellulose were 66.67% and 90.73%, respectively. The studies of structural characteristics showed that the changes in morphology caused by acid and enzymatic hydrolysis successively were significant in comparison to those of feedstock, resulting in increasing surface exposure and porosity. The disorganized morphological structure of AHR allows for greater accessibility to cellulase, which facilitates enzymatic hydrolysis. The spectrograms of FTIR and XRD suggested that the characteristic strength peaks for the corresponding typical functional groups of each component were changed and the crystallinity index of the solid residues were increased, compared with DG. In short, the saccharification process based on the combined hydrolysis of acid and enzyme to produce fermentable sugars was feasible and efficient.
关键词	Acids Cellulose Crystallinity Enzymes Fourier transform infrared spectroscopy Hydrolysis Liquid sugar Morphology Saccharification Sugars X ray diffraction Xylose Cellulose and hemicellulose Distiller's grains Enzymatic hydrolysis conditions Morphological structures Production and consumption Reducing sugars Scanning electronic microscopy (SEM) Structural characteristics
DOI	10.3969/j.issn.1002-6819.2013.05.032
收录类别	EI
语种	中文
出版者	Chinese Society of Agricultural Engineering
EI入藏号	20131616220349
EI主题词	Enzymatic hydrolysis
EI分类号	801 Chemistry - 802.2 Chemical Reactions - 803 Chemical Agents and Basic Industrial Chemicals - 804 Chemical Products Generally - 804.1 Organic Compounds - 811.3 Cellulose, Lignin and Derivatives - 822.3 Food Products - 951 Materials Science
来源库	Compendex
分类代码	801 Chemistry - 802.2 Chemical Reactions - 803 Chemical Agents and Basic Industrial Chemicals - 804 Chemical Products Generally - 804.1 Organic Compounds - 811.3 Cellulose, Lignin and Derivatives - 822.3 Food Products - 951 Materials Science
引用统计	无
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/113502
专题	能源与动力工程学院材料科学与工程学院生命科学与工程学院新能源学院
作者单位	1.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; 2.School of Energy and Power Engineering, Lanzhou University of Technology, Lanzhou 730050, China; 3.School of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; 4.Gansu Jinhui Liquor Company Limited, Longnan 742308, China
第一作者单位	生命科学与工程学院; 新能源学院
第一作者的第一单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Ren, Haiwei,Li, Jinping,Zhang, Yi,et al. Saccharification for distiller's grains based on combined hydrolysis with acid and enzyme[J]. Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering,2013,29(5):243-250.
APA	Ren, Haiwei.,Li, Jinping.,Zhang, Yi.,Li, Zhizhong.,Yin, Jianbo.,...&Shi, Jincai.(2013).Saccharification for distiller's grains based on combined hydrolysis with acid and enzyme.Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering,29(5),243-250.
MLA	Ren, Haiwei,et al."Saccharification for distiller's grains based on combined hydrolysis with acid and enzyme".Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering 29.5(2013):243-250.