IR
Characterization and catabolic genes analyses of oil-degrading Pseudomonas aeruginosa 1217
Yang Zhi1; Chen Jixiang2; Li Wenxin3; Hu Wei3; Sun Shangchen3; Li Yanlin1; Wang Yonggang1
2017
Source Publication微生物学杂志
ISSN1005-7021
Volume37Issue:6Pages:70-80
AbstractOne oil-degrading bacterial strain 1217 isolated from oil contaminated soil could degrade crude oil. It was identified and designated as Pseudomonas aeruginosaby morphology,physiology,biochemical and 16S rDNA sequence analyses. The strain grew well at 5-65 ℃ with the initial pH of 2-10 and NaCl concentrations of 0%-9%. It grew well in the medium containing different organic substrates as sole carbon sources,such as n-dodecane,n-octadecane,benzene,toluene,xylene and naphthaline. The degrading rates for hydrocarbons were 21.57% and 15.15% when it was cultured in minimal medium containing crude oil and different chain alkanes at 30 ℃ and 10 ℃ for 7 days. The bacterium produced biosurfactants with the surface tension reduction from 72.20 mN/m to 35.14 mN/m. The oil-degrading related genes such as alkane monooxygenase,toluene dioxygenase,biphenyl dioxygenase,ring-hydroxylating dioxygenase and oxidoreductase genes were detected in Pseudomonas aeruginosa 1217. The alkane monooxygenase and ringhydroxylating dioxygenase genes were further cloned and analyzed. The sequence similarities of the two genes with those of Pseudomonas aeruginosa PAO1 were 99.91% and 99.22% respectively. The isolated strain exhibits great potential for the bioremediation of the hydrocarbons contaminated environments.
KeywordOil-degradation Pseudomonas aeruginosa Identification Biosurfactant Degrading genes
Indexed ByCSCD
Language英语
WOS Research AreaMicrobiology
WOS SubjectMICROBIOLOGY
CSCD IDCSCD:6166699
Document Type期刊论文
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/73336
Collection兰州理工大学
Affiliation1.Coll. of Energy & Power Engin.,Lanzhou Uni. of Technol., Lanzhou, Gansu 730050, China
2.Schl. of Petrochem. Engin.,Lanzhou Uni. of Technol., Lanzhou, Gansu 730050, China
3.Lanzhou Lan Shi Eqpt. Engin. Res. Inst. Co. Ltd., Lanzhou, Gansu 730314, China
Recommended Citation
GB/T 7714
Yang Zhi,Chen Jixiang,Li Wenxin,et al. Characterization and catabolic genes analyses of oil-degrading Pseudomonas aeruginosa 1217[J]. 微生物学杂志,2017,37(6):70-80.
APA Yang Zhi.,Chen Jixiang.,Li Wenxin.,Hu Wei.,Sun Shangchen.,...&Wang Yonggang.(2017).Characterization and catabolic genes analyses of oil-degrading Pseudomonas aeruginosa 1217.微生物学杂志,37(6),70-80.
MLA Yang Zhi,et al."Characterization and catabolic genes analyses of oil-degrading Pseudomonas aeruginosa 1217".微生物学杂志 37.6(2017):70-80.
Files in This Item:
There are no files associated with this item.
Related Services
Usage statistics
Google Scholar
Similar articles in Google Scholar
[Yang Zhi]'s Articles
[Chen Jixiang]'s Articles
[Li Wenxin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang Zhi]'s Articles
[Chen Jixiang]'s Articles
[Li Wenxin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang Zhi]'s Articles
[Chen Jixiang]'s Articles
[Li Wenxin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.