Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1
Liu Yuanli1; Chen Jixiang1; Wang Yonggang2; Li Yanlin1; Cheng Lin1; Zhou Yongtao3
2016
Source Publication中国农业科技导报
ISSN1008-0864
Volume18Issue:6Pages:80-89
AbstractAn effective lipase-producing bacterium was isolated from soil samples of farmland in Lanzhou China,using Tween 80 agar plates. It was identified to belong to Rheinheimera sp. based on morphological and biochemical tests and 16S rDNA sequence analysis and was designated as Rheinheimera sp. NT-1. The cultivating conduction for the lipase production of Rheinheimera sp. NT-1 was studied in this paper. The optimum temperature, pH,salinity,liquid volume and incubation time for lipase production was 20℃,pH 8.0,8.1%(m/v),30 mL and 24 h,respectively. A Box-Behnken experimental design was used to optimize the lipase production. The optimization process parameters were pH 8.8 and salinity of 0.78% (m/v) at 32℃ through factorial design and RSM analysis. The highest lipase production was 7804.5 U/mL. The optimum temperature of the lipase was 60℃. The lipase showed relatively high specific activity at the range of 20~70℃ and pH 7~10. It might be a thermo stable and alkaline lipase. The enzyme could catalyze the synthesis of fatty acid ethyl esters such as ethyl oleate,ethyl palmitate and ethyl linoleate,which had potential applications in the biotransformation.
Keywordbiotransformation characterization isolation lipase Rheinheimera sp
Indexed ByCSCD
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBIOCHEMISTRY MOLECULAR BIOLOGY
CSCD IDCSCD:5868813
Document Type期刊论文
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/72249
Collection生命科学与工程学院
石油化工学院
Affiliation1.School of Petrochemical Engineering,Lanzhou University of Technology, Lanzhou, Gansu 730050, China
2.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
3.Petro China Beijing Gas Pipeline Co. Ltd., Beijing 100101, China
First Author AffilicationLanzhou University of Technology
First Signature AffilicationLanzhou University of Technology
Recommended Citation
GB/T 7714
Liu Yuanli,Chen Jixiang,Wang Yonggang,et al. Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1[J]. 中国农业科技导报,2016,18(6):80-89.
APA Liu Yuanli,Chen Jixiang,Wang Yonggang,Li Yanlin,Cheng Lin,&Zhou Yongtao.(2016).Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1.中国农业科技导报,18(6),80-89.
MLA Liu Yuanli,et al."Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1".中国农业科技导报 18.6(2016):80-89.
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