Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1

	Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1
	Liu Yuanli 1; Chen Jixiang1 ; Wang Yonggang2 ; Li Yanlin 1; Cheng Lin 1; Zhou Yongtao 3
	2016
发表期刊	中国农业科技导报
ISSN	1008-0864
卷号	18 期号:6 页码:80-89
摘要	An effective lipase-producing bacterium was isolated from soil samples of farmland in Lanzhou China,using Tween 80 agar plates. It was identified to belong to Rheinheimera sp. based on morphological and biochemical tests and 16S rDNA sequence analysis and was designated as Rheinheimera sp. NT-1. The cultivating conduction for the lipase production of Rheinheimera sp. NT-1 was studied in this paper. The optimum temperature, pH,salinity,liquid volume and incubation time for lipase production was 20℃,pH 8.0,8.1%(m/v),30 mL and 24 h,respectively. A Box-Behnken experimental design was used to optimize the lipase production. The optimization process parameters were pH 8.8 and salinity of 0.78% (m/v) at 32℃ through factorial design and RSM analysis. The highest lipase production was 7804.5 U/mL. The optimum temperature of the lipase was 60℃. The lipase showed relatively high specific activity at the range of 20~70℃ and pH 7~10. It might be a thermo stable and alkaline lipase. The enzyme could catalyze the synthesis of fatty acid ethyl esters such as ethyl oleate,ethyl palmitate and ethyl linoleate,which had potential applications in the biotransformation.
关键词	biotransformation characterization isolation lipase Rheinheimera sp
收录类别	CSCD
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	BIOCHEMISTRY MOLECULAR BIOLOGY
CSCD记录号	CSCD:5868813
引用统计
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/72249
专题	生命科学与工程学院石油化工学院
作者单位	1.School of Petrochemical Engineering,Lanzhou University of Technology, Lanzhou, Gansu 730050, China 2.School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China 3.Petro China Beijing Gas Pipeline Co. Ltd., Beijing 100101, China
第一作者单位	兰州理工大学
第一作者的第一单位	兰州理工大学
推荐引用方式 GB/T 7714	Liu Yuanli,Chen Jixiang,Wang Yonggang,et al. Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1[J]. 中国农业科技导报,2016,18(6):80-89.
APA	Liu Yuanli,Chen Jixiang,Wang Yonggang,Li Yanlin,Cheng Lin,&Zhou Yongtao.(2016).Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1.中国农业科技导报,18(6),80-89.
MLA	Liu Yuanli,et al."Isolation and Characterization of an Effective Thermostable Lipase Producing Rheinheimera sp. NT-1".中国农业科技导报 18.6(2016):80-89.