Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide

doi:10.1002/bab.1817

	Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide
	Chen, Zhangjun 1,2; Chen, Zhenbin1,2 ; Liu, Chunli1,2 ; Wang, Xudong 1,2; Zhou, Yongshan 1,2; Wang, Runtian 1,2
	2019-12-03
发表期刊	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
ISSN	0885-4513
卷号	66 期号:6 页码:990-998
摘要	In this work, TiO2, which was modified by glutaraldehyde, was adopted as the carrier; the penicillin G acylase (PGA) was immobilized and the influence of immobilized conditions, such as pH of solution, the concentration of PGA, the immobilization temperature, and the reaction time, on the catalytic performance of the immobilized PGA was investigated and optimized. During this process, potassium penicillin G (PG) was chosen as substrate, and the quantity of 6-aminopenicillanic acid (6-APA) produced by PG at the temperature of 25 degrees C for 3 Min in neutral solution was conscripted as the evaluation foundation, indexes, containing the loading capacity (ELC), the activity (EA), and activity retention rate (EAR), were calculated based on quantities of produced 6-APA and compared with finding out the suitable conditions. Results showed that when the solution pH, PGA concentration, immobilization temperature, and reaction time were 8.0, 2.5% (v/v), 35 degrees C, and 24 H, respectively, ELC, EA, and EAR presented optimal values of 9,190 U, 14,969 U/g, and 88.5% relatedly. After that, the stability and reusability of immobilized PGA were studied, and the results documented that the pH resistance, thermal stability, and storage stability of immobilized PGA were significantly improved. This work provided technique support for the practical application of immobilized PGA carrier.
关键词	TiO2 modified carrier penicillin G acylase immobilization
DOI	10.1002/bab.1817
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[51563015]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000500197200001
出版者	WILEY
EI入藏号	20195007817203
EI主题词	Drug products
EI分类号	452.4 Industrial Wastes Treatment and Disposal - 482.2 Minerals - 804.2 Inorganic Compounds
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/64251
专题	省部共建有色金属先进加工与再利用国家重点实验室材料科学与工程学院
通讯作者	Chen, Zhangjun; Liu, Chunli
作者单位	1.Lanzhou Univ Technol, Coll Mat Sci & Engn, Lanzhou, Gansu, Peoples R China; 2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou, Gansu, Peoples R China
第一作者单位	材料科学与工程学院; 省部共建有色金属先进加工与再利用国家重点实验室
通讯作者单位	材料科学与工程学院; 省部共建有色金属先进加工与再利用国家重点实验室
第一作者的第一单位	材料科学与工程学院
推荐引用方式 GB/T 7714	Chen, Zhangjun,Chen, Zhenbin,Liu, Chunli,et al. Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2019,66(6):990-998.
APA	Chen, Zhangjun,Chen, Zhenbin,Liu, Chunli,Wang, Xudong,Zhou, Yongshan,&Wang, Runtian.(2019).Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,66(6),990-998.
MLA	Chen, Zhangjun,et al."Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 66.6(2019):990-998.