Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide
Chen, Zhangjun1,2; Chen, Zhenbin1,2; Liu, Chunli1,2; Wang, Xudong1,2; Zhou, Yongshan1,2; Wang, Runtian1,2
2019-12-03
Source PublicationBIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
ISSN0885-4513
Volume66Issue:6Pages:990-998
AbstractIn this work, TiO2, which was modified by glutaraldehyde, was adopted as the carrier; the penicillin G acylase (PGA) was immobilized and the influence of immobilized conditions, such as pH of solution, the concentration of PGA, the immobilization temperature, and the reaction time, on the catalytic performance of the immobilized PGA was investigated and optimized. During this process, potassium penicillin G (PG) was chosen as substrate, and the quantity of 6-aminopenicillanic acid (6-APA) produced by PG at the temperature of 25 degrees C for 3 Min in neutral solution was conscripted as the evaluation foundation, indexes, containing the loading capacity (ELC), the activity (EA), and activity retention rate (EAR), were calculated based on quantities of produced 6-APA and compared with finding out the suitable conditions. Results showed that when the solution pH, PGA concentration, immobilization temperature, and reaction time were 8.0, 2.5% (v/v), 35 degrees C, and 24 H, respectively, ELC, EA, and EAR presented optimal values of 9,190 U, 14,969 U/g, and 88.5% relatedly. After that, the stability and reusability of immobilized PGA were studied, and the results documented that the pH resistance, thermal stability, and storage stability of immobilized PGA were significantly improved. This work provided technique support for the practical application of immobilized PGA carrier.
KeywordTiO2 modified carrier penicillin G acylase immobilization
DOI10.1002/bab.1817
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[51563015]
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000500197200001
PublisherWILEY
Document Type期刊论文
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/64251
Collection材料科学与工程学院
省部共建有色金属先进加工与再利用国家重点实验室
Corresponding AuthorChen, Zhenbin; Liu, Chunli
Affiliation1.Lanzhou Univ Technol, Coll Mat Sci & Engn, Lanzhou, Gansu, Peoples R China
2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou, Gansu, Peoples R China
First Author AffilicationColl Mat Sci & Engn;  State Key Laboratory Of Gansu Advanced Non-Ferrous Metal Materials
Corresponding Author AffilicationColl Mat Sci & Engn;  State Key Laboratory Of Gansu Advanced Non-Ferrous Metal Materials
First Signature AffilicationColl Mat Sci & Engn
Recommended Citation
GB/T 7714
Chen, Zhangjun,Chen, Zhenbin,Liu, Chunli,et al. Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2019,66(6):990-998.
APA Chen, Zhangjun,Chen, Zhenbin,Liu, Chunli,Wang, Xudong,Zhou, Yongshan,&Wang, Runtian.(2019).Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,66(6),990-998.
MLA Chen, Zhangjun,et al."Optimization of penicillin G acylase immobilized on glutaraldehyde-modified titanium dioxide".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 66.6(2019):990-998.
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