A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds
Zhang, Xin-guo; Sun, Qiao-Yun; Tang, Peng; Ma, Guo-Yan; Guo, Guang-Jun; Guo, Si-Jia; Ma, Xiao-Di
2020-04
发表期刊FOLIA MICROBIOLOGICA
ISSN0015-5632
卷号65期号:2页码:293-302
摘要Medicinal plants have been studied for potential endophytic interactions and numerous studies have provided evidence that seeds harbor diverse microbial communities, not only on their surfaces but also within the embryo. Adenosine deaminase (ADA) is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Therefore, in this study, 20 types of medicinal plant seeds were used to screen endophytic fungi with tissue homogenate and streak. In addition, 128 morphologically distinct endophyte strains were isolated and their ADA inhibitory activity determined by a spectrophotometric assay. The strain with the highest inhibitory activity was identified as Cochliobolus sp. Seven compounds were isolated from the strain using a chromatography method. Compound 3 showed the highest ADA inhibitory activity and was identified as 5-hydroxy-2-hydroxymethyl-4H-pyran-4-one, based on the results of 1H and 13C NMR spectroscopy. The results of molecular docking suggested that compound 3 binds to the active site and the nonspecific binding site of the ADA. Furthermore, we found that compound 3 is a mixed ADA inhibitor. These results indicate that endophytic strains are a promising source of ADA inhibitors and that compound 3 may be a superior source for use in the preparation of biologically active ADA inhibitor compounds used to treat cancer.
关键词Seed endophytes ADA inhibitors Molecular docking
DOI10.1007/s12223-019-00723-1
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[31860004] ; Gansu province natural science fund[17JR5RA128] ; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education (CN)[20180802]
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000517071000006
出版者SPRINGER
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://ir.lut.edu.cn/handle/2XXMBERH/64185
专题生命科学与工程学院
通讯作者Zhang, Xin-guo
作者单位Lanzhou Univ Technol, Sch Life Sci,Key Lab Herbal,Engn, Tebitan Drug Screening,Deep Proc Gansu Prov, Lanzhou, Peoples R China
第一作者单位生命科学与工程学院
通讯作者单位生命科学与工程学院
第一作者的第一单位生命科学与工程学院
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Zhang, Xin-guo,Sun, Qiao-Yun,Tang, Peng,et al. A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds[J]. FOLIA MICROBIOLOGICA,2020,65(2):293-302.
APA Zhang, Xin-guo.,Sun, Qiao-Yun.,Tang, Peng.,Ma, Guo-Yan.,Guo, Guang-Jun.,...&Ma, Xiao-Di.(2020).A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds.FOLIA MICROBIOLOGICA,65(2),293-302.
MLA Zhang, Xin-guo,et al."A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds".FOLIA MICROBIOLOGICA 65.2(2020):293-302.
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