A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds
Zhang, Xin-guo; Sun, Qiao-Yun; Tang, Peng; Ma, Guo-Yan; Guo, Guang-Jun; Guo, Si-Jia; Ma, Xiao-Di
2020-04
Source PublicationFOLIA MICROBIOLOGICA
ISSN0015-5632
Volume65Issue:2Pages:293-302
AbstractMedicinal plants have been studied for potential endophytic interactions and numerous studies have provided evidence that seeds harbor diverse microbial communities, not only on their surfaces but also within the embryo. Adenosine deaminase (ADA) is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Therefore, in this study, 20 types of medicinal plant seeds were used to screen endophytic fungi with tissue homogenate and streak. In addition, 128 morphologically distinct endophyte strains were isolated and their ADA inhibitory activity determined by a spectrophotometric assay. The strain with the highest inhibitory activity was identified as Cochliobolus sp. Seven compounds were isolated from the strain using a chromatography method. Compound 3 showed the highest ADA inhibitory activity and was identified as 5-hydroxy-2-hydroxymethyl-4H-pyran-4-one, based on the results of 1H and 13C NMR spectroscopy. The results of molecular docking suggested that compound 3 binds to the active site and the nonspecific binding site of the ADA. Furthermore, we found that compound 3 is a mixed ADA inhibitor. These results indicate that endophytic strains are a promising source of ADA inhibitors and that compound 3 may be a superior source for use in the preparation of biologically active ADA inhibitor compounds used to treat cancer.
KeywordSeed endophytes ADA inhibitors Molecular docking
DOI10.1007/s12223-019-00723-1
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31860004] ; Gansu province natural science fund[17JR5RA128] ; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education (CN)[20180802]
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000517071000006
PublisherSPRINGER
Citation statistics
Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.lut.edu.cn/handle/2XXMBERH/64185
Collection生命科学与工程学院
Corresponding AuthorZhang, Xin-guo
AffiliationLanzhou Univ Technol, Sch Life Sci,Key Lab Herbal,Engn, Tebitan Drug Screening,Deep Proc Gansu Prov, Lanzhou, Peoples R China
First Author AffilicationColl Life & Sci Engn
Corresponding Author AffilicationColl Life & Sci Engn
First Signature AffilicationColl Life & Sci Engn
Recommended Citation
GB/T 7714
Zhang, Xin-guo,Sun, Qiao-Yun,Tang, Peng,et al. A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds[J]. FOLIA MICROBIOLOGICA,2020,65(2):293-302.
APA Zhang, Xin-guo.,Sun, Qiao-Yun.,Tang, Peng.,Ma, Guo-Yan.,Guo, Guang-Jun.,...&Ma, Xiao-Di.(2020).A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds.FOLIA MICROBIOLOGICA,65(2),293-302.
MLA Zhang, Xin-guo,et al."A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds".FOLIA MICROBIOLOGICA 65.2(2020):293-302.
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