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A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds | |
Zhang, Xin-guo; Sun, Qiao-Yun; Tang, Peng; Ma, Guo-Yan; Guo, Guang-Jun; Guo, Si-Jia; Ma, Xiao-Di | |
2020-04 | |
发表期刊 | FOLIA MICROBIOLOGICA |
ISSN | 0015-5632 |
卷号 | 65期号:2页码:293-302 |
摘要 | Medicinal plants have been studied for potential endophytic interactions and numerous studies have provided evidence that seeds harbor diverse microbial communities, not only on their surfaces but also within the embryo. Adenosine deaminase (ADA) is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Therefore, in this study, 20 types of medicinal plant seeds were used to screen endophytic fungi with tissue homogenate and streak. In addition, 128 morphologically distinct endophyte strains were isolated and their ADA inhibitory activity determined by a spectrophotometric assay. The strain with the highest inhibitory activity was identified as Cochliobolus sp. Seven compounds were isolated from the strain using a chromatography method. Compound 3 showed the highest ADA inhibitory activity and was identified as 5-hydroxy-2-hydroxymethyl-4H-pyran-4-one, based on the results of 1H and 13C NMR spectroscopy. The results of molecular docking suggested that compound 3 binds to the active site and the nonspecific binding site of the ADA. Furthermore, we found that compound 3 is a mixed ADA inhibitor. These results indicate that endophytic strains are a promising source of ADA inhibitors and that compound 3 may be a superior source for use in the preparation of biologically active ADA inhibitor compounds used to treat cancer. |
关键词 | Seed endophytes ADA inhibitors Molecular docking |
DOI | 10.1007/s12223-019-00723-1 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[31860004] ; Gansu province natural science fund[17JR5RA128] ; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education (CN)[20180802] |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
WOS记录号 | WOS:000517071000006 |
出版者 | SPRINGER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/64185 |
专题 | 生命科学与工程学院 |
通讯作者 | Zhang, Xin-guo |
作者单位 | Lanzhou Univ Technol, Sch Life Sci,Key Lab Herbal,Engn, Tebitan Drug Screening,Deep Proc Gansu Prov, Lanzhou, Peoples R China |
第一作者单位 | 生命科学与工程学院 |
通讯作者单位 | 生命科学与工程学院 |
第一作者的第一单位 | 生命科学与工程学院 |
推荐引用方式 GB/T 7714 | Zhang, Xin-guo,Sun, Qiao-Yun,Tang, Peng,et al. A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds[J]. FOLIA MICROBIOLOGICA,2020,65(2):293-302. |
APA | Zhang, Xin-guo.,Sun, Qiao-Yun.,Tang, Peng.,Ma, Guo-Yan.,Guo, Guang-Jun.,...&Ma, Xiao-Di.(2020).A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds.FOLIA MICROBIOLOGICA,65(2),293-302. |
MLA | Zhang, Xin-guo,et al."A new mixed inhibitor of adenosine deaminase produced by endophytic Cochliobolus sp. from medicinal plant seeds".FOLIA MICROBIOLOGICA 65.2(2020):293-302. |
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