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| Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate | |
Wang, Yonggang1,2 ; Jing, Yanjun1,2; Leng, Feifan1,2; Wang, Shiwei1,2; Wang, Fan1,2; Zhuang, Yan1,2; Liu, Xiaofeng1,2; Wang, Xiaoli3; Ma, Xueqing4
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| 2017-08 | |
| 发表期刊 | SUGAR TECH
 |
| ISSN | 0972-1525 |
| 卷号 | 19期号:4页码:424-431 |
| 摘要 | To obtain maximal linear regression correlation coefficient (R-2) of glucose standard curve determinations, single-factor test and response surface methodology were applied to optimize conditions including reaction time, incubation temperature and phenol concentration. For investigating optimization of this method, the effects of different metal ions, amino acids and proteins, respectively, were evaluated. The contents of several polysaccharides were determined by three methods, and it was found that the absorption value at 490 nm of the reaction system (230 mu L) placed in the microplate involving 150 mu L sulfuric acid and 30 mu L 5 % phenol solution was in linear relationship with the concentration of glucose range from 10 to 100 nmol/well at 85 degrees C for 20 min. Our results also suggested that the method had a relative deviation of 0.4 %, and the recovery was between 102.7 and 104.10 %. |
| 关键词 | Microplate Phenol-sulfuric acid colorimetry Glucose Response surface methodology Methodological evaluation |
| DOI | 10.1007/s12355-016-0473-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Foundation of Excellent Young Teachers of LUT[10-061406] |
| WOS研究方向 | Agriculture |
| WOS类目 | Agronomy |
| WOS记录号 | WOS:000403439900012 |
| 出版者 | SPRINGER INDIA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/33179 |
| 专题 | 生命科学与工程学院 |
| 通讯作者 | Wang, Yonggang |
| 作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, 287 Langongping, Lanzhou 730050, Gansu, Peoples R China 2.Gansu Educ Dept, Key Lab Screening Evaluat & Adv Proc TCM & Tibeta, Lanzhou 730050, Peoples R China 3.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Peoples R China 4.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China |
| 第一作者单位 | 生命科学与工程学院 |
| 通讯作者单位 | 生命科学与工程学院 |
| 第一作者的第一单位 | 生命科学与工程学院 |
| 推荐引用方式 GB/T 7714 | Wang, Yonggang,Jing, Yanjun,Leng, Feifan,et al. Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate[J]. SUGAR TECH,2017,19(4):424-431. |
| APA | Wang, Yonggang.,Jing, Yanjun.,Leng, Feifan.,Wang, Shiwei.,Wang, Fan.,...&Ma, Xueqing.(2017).Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate.SUGAR TECH,19(4),424-431. |
| MLA | Wang, Yonggang,et al."Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate".SUGAR TECH 19.4(2017):424-431. |
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