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| Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis | |
Wang, Yonggang1 ; Li, Yuanli1; Ma, Xueqing2; Ren, Haiwei1; Fan, Wenguang1; Leng, Feifan1; Yang, Mingjun1; Wang, Xiaoli3
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| 2018-10-15 | |
| 发表期刊 | INDUSTRIAL CROPS AND PRODUCTS
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| ISSN | 0926-6690 |
| 卷号 | 122页码:596-608 |
| 摘要 | Glycyrrhiza uralensis is an important traditional Chinese herbal medicine. Polysaccharide is one of active components of G. uralensis. In this paper, the extraction conditions of crude polysaccharides from G. uralensis (GPsO) were optimized by central composite design (CCD), and Three purified polysaccharide fractions (GPs1, GPs2 and GPs3) were studied based on physicochemical property, monosaccharide composition, molecular weight, structures characteristics and antioxidant activities. Results showed that the yield of GPsO reached at the maximum of 4.23%, when the optimum extraction process was 1:13 g/mL (solid-liquid ratio), 600 W (ultrasonic power) at 70 degrees C for 85 min. Except for GPs2, the others (GPsO, GPs1 and GPs3) are non-reducing sugars. They don't contain starch. GPsO and GPs1 contain protein. The molecular weight, monosaccharide composition and Fourier transform infrared spectroscopy (FT-IR) assay showed that GPs1, GPs2 and GPs3 are pyran polysaccharides with a weight average molecular weight (Mw) of 4.513 x 10(3), 1.378 x 10(5) and 2.084 x 10(5) g/mol, respectively. GPs1, GPs2 and GPs3 mainly contain glucose and galactose, respectively. Scanning electron microscope (SEM) demonstrated that GPs1, GPs2 and GPs3 are flaky with a pore structure. Atomic force microscopy (AFM) displayed GPs1 and GPs2 have a large number of dispersions and a small amount of smaller globular aggregates, while GPs3 is the opposite of the abovementioned result. Besides, the antioxidant activity assay of these polysaccharides illustrated that GPs1 has a strong scavenging effect on DPPH radicals and superoxide radical. |
| 关键词 | Polysaccharides Optimization Characterization Antioxidant activity |
| DOI | 10.1016/j.indcrop.2018.06.011 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31460032] ; National Natural Science Foundation of China[81660581] |
| WOS研究方向 | Agriculture |
| WOS类目 | Agricultural Engineering ; Agronomy |
| WOS记录号 | WOS:000442067300071 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://ir.lut.edu.cn/handle/2XXMBERH/32396 |
| 专题 | 生命科学与工程学院 |
| 通讯作者 | Wang, Yonggang; Leng, Feifan |
| 作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd 287, Lanzhou 730050, Gansu, Peoples R China 2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab, 1 Xujiaping, Lanzhou 730046, Gansu, Peoples R China 3.Lanzhou Inst Husb & Pharmaceut Sci CAAS, Lanzhou 730050, Gansu, Peoples R China |
| 第一作者单位 | 生命科学与工程学院 |
| 通讯作者单位 | 生命科学与工程学院 |
| 第一作者的第一单位 | 生命科学与工程学院 |
| 推荐引用方式 GB/T 7714 | Wang, Yonggang,Li, Yuanli,Ma, Xueqing,et al. Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis[J]. INDUSTRIAL CROPS AND PRODUCTS,2018,122:596-608. |
| APA | Wang, Yonggang.,Li, Yuanli.,Ma, Xueqing.,Ren, Haiwei.,Fan, Wenguang.,...&Wang, Xiaoli.(2018).Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis.INDUSTRIAL CROPS AND PRODUCTS,122,596-608. |
| MLA | Wang, Yonggang,et al."Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis".INDUSTRIAL CROPS AND PRODUCTS 122(2018):596-608. |
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