Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin

doi:10.1016/j.scienta.2023.112296

	Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin
	Wang, Jinbin 1,3; Yue, Tong 3; He, Chuan 1,2; Zhou, Yifan 1; Bai, Yinshuang 1; Li, Qingwei 1; Jiang, Wei 1,2; Huang, Yanna 1; Liu, Xiaofeng3
	2023-11-01
在线发表时间	2023-06
发表期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
卷号	321
摘要	Bacillus subtilis GSJB-1210 with strong antagonism against Ralstonia solanacearum was combined with different bactericides to investigate its ability to mitigate tomato bacterial wilt. The control activity of six bactericides and GSJB-1210 against tomato bacterial wilt was determined via the bacteriostasis assay, and the concentration for 50% of maximal effect (EC50) value was calculated. The compatibility of the bactericide and GSJB-1210 was determined by ultraviolet spectrophotometry, and the compound scheme was determined by the Horsfall method. The EC50 of ningnanmycin was 3.66 mg/L and it had good biocompatibility with the GSJB-1210 strain. When ningnanmycin (3.66 mg/L) was combined with GSJB-1210 (1.0 x 108 CFU/mL) at a volume ratio of 4:6, the mitigation effect on tomato bacterial wilt reached 77.02%, which was significantly higher than that of the individual agents. To determine the impact of GSJB-1210 on the tomato rhizosphere microbial communities, the V3-V4 regions of 16S rRNA were amplified. The & alpha;- and & beta;-diversity of tomato rhizosphere bacteria were increased and the relative abundance of potential beneficial bacteria at genus level (Pseudomonas and Burkholderia) was improved. Ningnanmycin can be combined with B. subtilis GSJB-1210 to control bacterial wilt, with a significant synergistic effect. Mixed application can reduce the required amount of chemical pesticides by half. This combination can be used as a novel biocontrol agent to attain sustainability in disease management due to its intense antibacterial activity and ability to transform the microbial community structure toward a healthy state.
关键词	Tomato bacterial wilt Bactericides Bacillus subtilis Ningnanmycin Combined treatment
DOI	10.1016/j.scienta.2023.112296
收录类别	SCIE
语种	英语
WOS研究方向	Agriculture
WOS类目	Horticulture
WOS记录号	WOS:001025503600001
出版者	ELSEVIER
原始文献类型	Article
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/163730
专题	生命科学与工程学院
通讯作者	Huang, Yanna
作者单位	1.Shanghai Acad Agr Sci, Inst Biotechnol Res, 2901 Beidi Rd, Shanghai 201106, Peoples R China; 2.Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
第一作者单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Wang, Jinbin,Yue, Tong,He, Chuan,et al. Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin[J]. SCIENTIA HORTICULTURAE,2023,321.
APA	Wang, Jinbin.,Yue, Tong.,He, Chuan.,Zhou, Yifan.,Bai, Yinshuang.,...&Liu, Xiaofeng.(2023).Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin.SCIENTIA HORTICULTURAE,321.
MLA	Wang, Jinbin,et al."Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin".SCIENTIA HORTICULTURAE 321(2023).