Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method

doi:10.1016/j.ultsonch.2021.105552

	Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method
	Wang, Yonggang1 ; Sun, Shangchen 2; Yu, Linmiao 1; Hu, Shu 1; Fan, Wenguang1 ; Leng, Feifan1 ; Ma, Jianzhong1
	2021-06
发表期刊	Ultrasonics Sonochemistry
ISSN	1350-4177
卷号	74
摘要	As a basic technique of molecular cloning, bio-transformation has been successfully used in the fields of biomedicine and food processing. In this study, we established a transformation system of exogenous DNA into E. coli cells mediated by ultrasound. Under the optimal conditions (i.e. 35 °C, 40 W, 25 s, OD600 = 0.4–0.6) optimized by RSM, the transformation efficiency reached at 1.006 × 107 CFU/μg DNA. The results of membrane permeability, macromolecular substance and cell structure analysis before and after ultrasound treatment showed that the damage of host cells induced by lower (40 W) ultrasound and shorter ultrasound time (25 s) was reversible, and the transformation efficiency and cell survival rate were not significantly affected under this condition. In brief, proper changes in cell membrane and cell wall were the basic conditions for host cells to uptake exogenous DNA, while, whether exogenous DNA could be replicated and expressed in cells depends on the viability of host cells. © 2021 The Author(s)
关键词	Clone cells Cytology Efficiency Escherichia coli Food processing Ultrasonic applications Chemical method Genetic transformation Host cells Molecular cloning Optimisations Plasmid puc19 Transformation efficiency Transformation systems Treatment methods Ultrasound treatments
DOI	10.1016/j.ultsonch.2021.105552
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Acoustics ; Chemistry
WOS类目	Acoustics ; Chemistry, Multidisciplinary
WOS记录号	WOS:000663409800004
出版者	Elsevier B.V.
EI入藏号	20211610236609
EI主题词	DNA
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 753.3 Ultrasonic Applications ; 822.2 Food Processing Operations ; 913.1 Production Engineering
来源库	WOS
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.lut.edu.cn/handle/2XXMBERH/148418
专题	生命科学与工程学院
通讯作者	Wang, Yonggang; Leng, Feifan; Ma, Jianzhong
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Peoples R China
第一作者单位	生命科学与工程学院
通讯作者单位	生命科学与工程学院
第一作者的第一单位	生命科学与工程学院
推荐引用方式 GB/T 7714	Wang, Yonggang,Sun, Shangchen,Yu, Linmiao,et al. Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method[J]. Ultrasonics Sonochemistry,2021,74.
APA	Wang, Yonggang.,Sun, Shangchen.,Yu, Linmiao.,Hu, Shu.,Fan, Wenguang.,...&Ma, Jianzhong.(2021).Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method.Ultrasonics Sonochemistry,74.
MLA	Wang, Yonggang,et al."Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method".Ultrasonics Sonochemistry 74(2021).